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EXOC6 Polyclonal antibody

EXOC6 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12723-1-AP

Synonyms

SEC15L, SEC15A, SEC15 like protein 1, SEC15, Exocyst complex component 6

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human testis tissue using EXOC6 Polyclonal antibody (12723-1-AP)

    WB analysis of human testis using 12723-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 12723-1-AP (EXOC6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using EXOC6 Polyclonal antibody (12723-1-AP)

    WB analysis of human testis using 12723-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 12723-1-AP (EXOC6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EXOC6 Polyclonal antibody (12723-1-AP)

    IF Staining of HepG2 using 12723-1-AP

    Immunofluorescent analysis of HepG2 cells, using EXOC6 antibody 12723-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inhuman testis tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12723-1-AP targets EXOC6 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EXOC6 fusion protein Ag3418
Full Name exocyst complex component 6
Calculated Molecular Weight 804 aa, 94 kDa
Observed Molecular Weight94 kDa, 116 kDa
GenBank Accession NumberBC028395
Gene Symbol EXOC6
Gene ID (NCBI) 54536
RRIDAB_10734442
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EXOC6 antibody 12723-1-APDownload protocol
IF protocol for EXOC6 antibody 12723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

IP6-assisted CSN-COP1 competition regulates a CRL4-ETV5 proteolytic checkpoint to safeguard glucose-induced insulin secretion.

Authors - Hong Lin
View Article
humanWB,IF

Placenta

Exocyst complex protein expression in the human placenta.

Authors - I M Gonzalez
View Article
humanWB

Development

The exocyst functions in niche cells to promote germline stem cell differentiation by directly controlling EGFR membrane trafficking.

Authors - Ying Mao
View Article