Anticorps Polyclonal de lapin anti-SEC22B

• Phare
• Validé par KD/KO

SEC22B Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 14776-1-AP

Synonymes

ER Golgi SNARE of 24 kDa, ERS 24, ERS24, SEC22B, SEC22L1

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Galerie de données de validation

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  WB analysis using 14776-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14776-1-AP (SEC22B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14776-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14776-1-AP (SEC22B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14776-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14776-1-AP (SEC22B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 14776-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14776-1-AP (SEC22B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 14776-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14776-1-AP (SEC22B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14776-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14776-1-AP (SEC22B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14776-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14776-1-AP (SEC22B antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 14776-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14776-1-AP (SEC22B antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of MCF-7 using 14776-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 14776-1-AP (SEC22B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

14776-1-AP cible SEC22B dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SEC22B Protéine recombinante Ag6077
• Nom complet: SEC22 vesicle trafficking protein homolog B (S. cerevisiae)
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC001364
• Symbole du gène: SEC22B
• Identification du gène (NCBI): 9554
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor (SNARE) proteins are critical components of the membrane fusion machinery (PMID: 20163564). SEC22B is a SNARE involved in endoplasmic reticulum (ER)/Golgi membrane trafficking. SEC22B localizes to the ER-Golgi intermediate compartment (ERGIC) and has been identified as a regulator of antigen crosspresentation (PMID: 22153078).

Publications

Species	Application	Title
human	WB	Cell Metab; Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.; Authors - Xiao Wang
mouse	WB,IF	Nat Cell Biol; The SNARE Sec22b has a non-fusogenic function in plasma membrane expansion.; Authors - Maja Petkovic
		Sci Adv; Integrated proteogenetic analysis reveals the landscape of a mitochondrial-autophagosome synapse during PARK2-dependent mitophagy.; Authors - Jin-Mi Heo
mouse	WB	J Neurosci; Unbalanced regulation of Sec22b and Ykt6 blocks autophagosome axonal retrograde flux in neuronal ischemia-reperfusion injury.; Authors - Haiying Li ; KD Validated
rat	WB,IF	Brain Res Bull; Upregulation of Sec22b plays a neuroprotective role in a rat model of traumatic brain injury via inducing protective autophagy.; Authors - Di Li ; KD Validated

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