GOSR2/Membrin Monoclonal antibody

• Featured Product
• KD/KO Validated

GOSR2/Membrin Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

7E7C7

Cat no : 66134-1-Ig

Synonyms

27 kDa Golgi SNARE protein, Bos1, GOSR2, GS27, Membrin

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Validation Data Gallery

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  WB analysis of Jurkat using 66134-1-Ig

  WB result of GOSR2/Membrin antibody (66134-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOSR2/Membrin transfected Jurkat cells.

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  WB analysis using 66134-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66134-1-Ig (GOSR2/Membrin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 66134-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66134-1-Ig (GOSR2/Membrin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66134-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66134-1-Ig (GOSR2/Membrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66134-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66134-1-Ig (GOSR2/Membrin antibody at dilution of 1:50.

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  IHC staining of human liver using 66134-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66134-1-Ig (GOSR2/Membrin antibody at dilution of 1:50.

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  IF Staining of human liver cancer using 66134-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using GOSR2/Membrin antibody (66134-1-Ig, Clone: 7E7C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HSC-T6 cells, Jurkat cells, PC-12 cells, HEK-293 cells, HepG2 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 66134-1-Ig is GOSR2/Membrin Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: GOSR2/Membrin fusion protein Ag21393
• Full Name: golgi SNAP receptor complex member 2
• Calculated Molecular Weight: 212 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC009710
• Gene Symbol: Membrin
• Gene ID (NCBI): 9570
• RRID: AB_2881533
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Golgi SNAP receptor complex member 2 (GOSR2), also known as GS27 or Membrin, is a member of the solube NSF attachment protein receptor (SNARE) family of vesicle docking proteins. GOSR2 is localized in Golgi apparatus. It is involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network.

Publications

Species	Application	Title
human	WB	J Cell Sci; Neutral sphingomyelinase 2 controls exosome secretion by counteracting V-ATPase-mediated endosome acidification.; Authors - Dolma Choezom