GOSR2/Membrin Polyclonal antibody

• Featured Product
• KD/KO Validated

GOSR2/Membrin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12095-1-AP

Synonyms

Membrin, GS27, GOSR2, Golgi SNAP receptor complex member 2, 27 kDa Golgi SNARE protein

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Validation Data Gallery

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  WB analysis of Jurkat using 12095-1-AP

  WB result of GOSR2/Membrin antibody (12095-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOSR2/Membrin transfected Jurkat cells.

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  WB analysis using 12095-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12095-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse stomach using 12095-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12095-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 12095-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12095-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOSR2/Membrin antibody (12095-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HepG2 using 12095-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12095-1-AP

  Immunofluorescent analysis of () fixed HepG2 cells using 12095-1-AP (GOSR2/Membrin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: Jurkat cells, human liver tissue, mouse liver tissue, mouse stomach tissue, L02 cells, rat liver tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12095-1-AP targets GOSR2/Membrin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GOSR2/Membrin fusion protein Ag2736
• Full Name: golgi SNAP receptor complex member 2
• Calculated Molecular Weight: 212 aa, 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC009710
• Gene Symbol: Membrin
• Gene ID (NCBI): 9570
• RRID: AB_2113610
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Golgi SNAP receptor complex member 2 (GOSR2), also known as GS27 or Membrin, is a member of the solube NSF attachment protein receptor (SNARE) family of vesicle docking proteins. GOSR2 is localized in Golgi apparatus. It is involved in transport of proteins from the cis/medial-Golgi to the trans-Golgi network.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Lysosomal exocytosis releases pathogenic α-synuclein species from neurons in synucleinopathy models; Authors - Ying Xue Xie
	WB	Commun Biol; Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner.; Authors - Xihua Yue