USE1 Polyclonal antibody

• Featured Product
• KD/KO Validated

USE1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 25218-1-AP

Synonyms

MDS032, P31, SLT1, USE1, USE1 like protein, USE1L, Vesicle transport protein USE1

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Validation Data Gallery

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  WB analysis of HEK-293 using 25218-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25218-1-AP (USE1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25218-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25218-1-AP (USE1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 25218-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25218-1-AP (USE1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 25218-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25218-1-AP (USE1 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells
• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

The immunogen of 25218-1-AP is USE1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USE1 fusion protein Ag18587
• Full Name: unconventional SNARE in the ER 1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 259 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC008455
• Gene Symbol: USE1
• Gene ID (NCBI): 55850
• RRID: AB_2879966
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USE1(Vesicle transport protein USE1) is also named as USE1L, p31 and belongs to the USE1 family. It may be an essential molecule involved in the maintenance of ER morphology (and its deficiency leads to ER stress-induced apoptosis). USE1 also participates in the degradative function of lysosomes. This protein has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	PLoS Pathog; SNARE protein USE1 is involved in the glycosylation and the expression of mumps virus fusion protein and important for viral propagation; Authors - Yaqing Liu ; KD Validated
human	WB	J Cell Sci; A trap mutant reveals the physiological client spectrum of TRC40.; Authors - Javier Coy-Vergara