Anticorps Polyclonal de lapin anti-RTN3
RTN3 Polyclonal Antibody for WB, IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain et plus (3)
Applications
WB, IP, IF, ELISA
Conjugaison
Non conjugué
N° de cat : 12055-2-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules HEK-293T, cellules HeLa, cellules MG U-87, cellules U2OS, tissu cérébral humain |
| Résultats positifs en IP | cellules HeLa |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 16 publications below |
| IF | See 5 publications below |
| IP | See 2 publications below |
Informations sur le produit
12055-2-AP cible RTN3 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Réactivité citée | rat, Humain, singe, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | RTN3 Protéine recombinante Ag2685 |
| Nom complet | reticulon 3 |
| Masse moléculaire calculée | 113 kDa |
| Poids moléculaire observé | 24 kDa, 100-113 kDa |
| Numéro d’acquisition GenBank | BC011394 |
| Symbole du gène | RTN3 |
| Identification du gène (NCBI) | 10313 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). Four mammalian reticulons (RTN1-4) exist. Reticulon3 (RTN3) is highly expressed in neurons. RTN3 has been shown to modulate secretory pathway protein trafficking. It is a negative modulator of BACE1 (β-secretase) proteolytic activity. RTN3 may induce caspase-8 cascade and apoptosis and may favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for RTN3 antibody 12055-2-AP | Download protocol |
| IP protocol for RTN3 antibody 12055-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Protein Cell Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice | ||
Acta Neuropathol Integrative proteomics highlight presynaptic alterations and c-Jun misactivation as convergent pathomechanisms in ALS | ||
J Pineal Res Melatonin restores endoplasmic reticulum homeostasis to protect injured neurons in a rat model of chronic cervical cord compression | ||
Dev Cell TAOK2 is an ER-localized kinase that catalyzes the dynamic tethering of ER to microtubules. | ||
Acta Neuropathol Commun Liver X receptor-agonist treatment rescues degeneration in a Drosophila model of hereditary spastic paraplegia. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Chun (Verified Customer) (09-07-2020) | This is a very good antibody for immunoblotting.
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