RTN4/NOGO Polyclonal antibody

• Featured Product
• KD/KO Validated

RTN4/NOGO Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 10950-1-AP

Synonyms

NOGO, RTN4, Nbla10545, Nbla00271, My043

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Validation Data Gallery

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  WB analysis using 10950-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10950-1-AP (RTN4/NOGO antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10950-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10950-1-AP (RTN4/NOGO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10950-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10950-1-AP (RTN4/NOGO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10950-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10950-1-AP (RTN4/NOGO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 10950-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10950-1-AP (RTN4/NOGO antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10950-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10950-1-AP (RTN4/NOGO antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of A375 using 10950-1-AP

  Immunofluorescent analysis of A375 cells using 10950-1-AP (RTN4/NOGO antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 10950-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using RTN4/NOGO antibody (10950-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 10950-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RTN4/NOGO (10950-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: A375 cells, HepG2 cells, A549 cells, SH-SY5Y cells
• Positive IHC detected in: mouse brain tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells, A375 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 3 publications below

Product Information

10950-1-AP targets RTN4/NOGO in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RTN4/NOGO fusion protein Ag1392
• Full Name: reticulon 4
• Calculated Molecular Weight: 130 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC012619
• Gene Symbol: NOGO
• Gene ID (NCBI): 57142
• RRID: AB_2877741
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. RTN4 (also known as Neurite outgrowth inhibitor or Nogo) is a myelin-associated neurite growth inhibitory protein. Some isoforms of RTN4 have been described. RTN4A (Nogo-A, runs at ~200 kDa), RTN4B (Nogo-B, 40-55 kDa), and RTN4C (Nogo-C, 22-25 kDa) are three major isoforms (PMID: 31092426; 16469703).

Publications

Species	Application	Title
human	WB	PLoS Biol; Reticulon and CLIMP-63 regulate nanodomain organization of peripheral ER tubules.; Authors - Guang Gao ; KD Validated
human	WB,IF	Autophagy; Dengue and Zika viruses subvert reticulophagy by NS2B3-mediated cleavage of FAM134B.; Authors - Nicholas J Lennemann
human	WB	J Cell Sci; SEC24A facilitates colocalization and calcium flux between endoplasmic reticulum and mitochondria.; Authors - Tamutenda Chidawanyika
rat	WB	Drug Des Devel Ther; Tanshinone IIA Promotes Axonal Regeneration in Rats with Focal Cerebral Ischemia Through the Inhibition of Nogo-A/NgR1/RhoA/ROCKII/MLC Signaling.; Authors - Jing Wang
mouse	WB	Neurol Res; Erythropoietin attenuates axonal injury after middle cerebral artery occlusion in mice.; Authors - Rongliang Wang
mouse	WB	NPJ Parkinsons Dis; Deficiency of Perry syndrome-associated p150Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities; Authors - Jia Yu