RTN4/NOGO Polyclonal antibody

RTN4/NOGO Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 10740-1-AP

Synonyms

NOGO, RTN4, Nbla10545, Nbla00271, My043

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Validation Data Gallery

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  WB analysis using 10740-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10740-1-AP (RTN4/NOGO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10740-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10740-1-AP (RTN4/NOGO antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 10740-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10740-1-AP (RTN4/NOGO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10740-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10740-1-AP (RTN4/NOGO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10740-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10740-1-AP (RTN4/NOGO antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of A549 using 10740-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RTN4/NOGO antibody (10740-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse testis tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 17 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

10740-1-AP targets RTN4/NOGO in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RTN4/NOGO fusion protein Ag1164
• Full Name: reticulon 4
• Calculated Molecular Weight: 130 kDa
• Observed Molecular Weight: 190-210 kDa, 45 kDa, 22-25 kDa
• GenBank Accession Number: BC007109
• Gene Symbol: NOGO
• Gene ID (NCBI): 57142
• RRID: AB_2183598
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER) (PMID: 18177508). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). They are involved in shaping the tubular endoplasmic reticulum network, membrane trafficking, inhibition of axonal growth, and apoptosis (PMID: 24218324). Four mammalian reticulons (RTN1-4) exist. RTN4 (also known as Neurite outgrowth inhibitor or Nogo) is a myelin-associated neurite growth inhibitory protein. Some isoforms of RTN4 have been described. RTN4A (Nogo-A, runs at ~200 kDa), RTN4B (Nogo-B, 40-55 kDa), and RTN4C (Nogo-C, 22-25 kDa) are three major isoforms (PMID: 31092426; 16469703).

Publications

Species	Application	Title
human	WB	Nature; ER proteins decipher the tubulin code to regulate organelle distribution; Authors - Pengli Zheng
mouse	WB	Cell Metab; Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.; Authors - Xiao Wang
human,mouse	WB,IF	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
human	WB	Autophagy; DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B.; Authors - Xiaoyu Tian
human	WB	Autophagy; SARS-CoV-2 ORF3a induces RETREG1/FAM134B-dependent reticulophagy and triggers sequential ER stress and inflammatory responses during SARS-CoV-2 infection.; Authors - Xiaolin Zhang
human	WB,IF	Acta Neuropathol Commun; Liver X receptor-agonist treatment rescues degeneration in a Drosophila model of hereditary spastic paraplegia.; Authors - Dwayne J Byrne