Caspase 8/p43/p18 Monoclonal antibody

• Featured Product
• KD/KO Validated

Caspase 8/p43/p18 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (6)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

80

CloneNo.

2B9H8

Cat no : 66093-1-Ig

Synonyms

CASP8, Caspase 8, CASP-8, CAP4, Apoptotic protease Mch-5

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Validation Data Gallery

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  WB analysis of Jurkat using 66093-1-Ig

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66093-1-Ig (Caspase 8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa cells using 66093-1-Ig

  WB result of CASP8 with normal and apotosis Hela cell. P18 can be get in the apotosis cell. 60kd,50kd and 45kd bands are some isoforms of precursor CASP8.

• 

  WB analysis using 66093-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66093-1-Ig (Caspase 8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66093-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66093-1-Ig (CASP8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66093-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66093-1-Ig (CASP8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 66093-1-Ig

  IP result of anti-Caspase 8/p43/p18 (IP:66093-1-Ig, 5ug; Detection:66093-1-Ig 1:500) with HepG2 cells lysate 2400ug.

• 

  IHC staining of human liver cancer using 66093-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66093-1-Ig (Caspase 8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66093-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66093-1-Ig (Caspase 8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 66093-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66093-1-Ig (CASP8 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver using 66093-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66093-1-Ig (CASP8 Antibody) at dilution of 1:50.

• 

  IF Staining of HeLa using 66093-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 8/p43/p18 antibody (66093-1-Ig, Clone: 2B9H8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, HepG2 cells, HEK-293 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 71 publications below
• IHC: See 5 publications below
• IF: See 6 publications below
• IP: See 2 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Product Information

66093-1-Ig targets Caspase 8/p43/p18 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig, monkey, chicken, sheep
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Caspase 8/p43/p18 fusion protein Ag20524
• Full Name: caspase 8, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 538 aa, 62 kDa
• Observed Molecular Weight: 53-57 kDa, 32-45 kDa, 18 kDa
• GenBank Accession Number: BC028223
• Gene Symbol: Caspase 8
• Gene ID (NCBI): 841
• RRID: AB_11232214
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3 (PMID:21816816). It may control diabetic embryopathy-associated apoptosis via regulation of the Bid-stimulated mitochondrion/caspase-9 pathway (PMID:19194987). Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro- and cleaved-caspase 8.

Publications

Species	Application	Title
human	WB	Cancer Commun (Lond); Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies; Authors - Lu Tang
human	WB	Mol Cell; Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover.; Authors - Dianrong Li ; KO Validated
human	WB,IHC	Acta Biomater; TRAIL-modified, doxorubicin-embedded periodic mesoporous organosilica nanoparticles for targeted drug delivery and efficient antitumor immunotherapy.; Authors - Xinyao Feng
mouse	WB	J Pineal Res; Melatonin alleviates weanling stress in mice: involvement of intestinal microbiota.; Authors - Wenkai Ren
human	WB,IF	Chem Sci; Integration of exonuclease III-powered three-dimensional DNA walker with single-molecule detection for multiple initiator caspases assay.; Authors - Meng Liu
chicken	WB	J Hazard Mater; The role of ATF6 in Cr(VI)-induced apoptosis in DF-1 cells.; Authors - Shuo Zhang

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