BACE1 Polyclonal antibody

• Featured Product
• KD/KO Validated

BACE1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

37

Reviews (1)

Cat no : 12807-1-AP

Synonyms

Beta-site amyloid precursor protein cleaving enzyme 1, Beta-secretase 1, Beta secretase 1, Aspartyl protease 2, ASP21

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Validation Data Gallery

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  WB analysis of mouse brain using 12807-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12807-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12807-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12807-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of zebrafish using 12807-1-AP

  zebrafish tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12807-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12807-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 12807-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12807-1-AP (BACE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12807-1-AP

  IP result of anti-BACE1 (IP:12807-1-AP, 3ug; Detection:12807-1-AP 1:500) with mouse brain tissue lysate 5000ug.

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  IP experiment using 12807-1-AP

  IP result of BACE1 antibody (12807-1-AP for IP and Detection) with mouse brain tissue lysate.

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  IHC staining of mouse brain using 12807-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12807-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12807-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12807-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12807-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 12807-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12807-1-AP (BACE1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse brain using 12807-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BACE1 antibody (12807-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, mouse pancreas tissue, HeLa cells, zebrafish tissue, PC-12 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 35 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Product Information

12807-1-AP targets BACE1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

• Tested Reactivity: human, mouse, rat, zebrafish
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BACE1 fusion protein Ag3559
• Full Name: beta-site APP-cleaving enzyme 1
• Calculated Molecular Weight: 501 aa, 56 kDa
• Observed Molecular Weight: 70-75 kDa, 42-56 kDa
• GenBank Accession Number: BC036084
• Gene Symbol: BACE1
• Gene ID (NCBI): 23621
• RRID: AB_10638760
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

beta-secretase, encoded by BACE1(beta-stie amyloid beta A4 precursor protein-cleavage enzyem1) gene, is a neuron-specific membrane-associated protease that participate in the two step proteolytic processing of the APP. Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has a high expression in the brain and pancreas. This protein has 6 isoforms produced by alternative splicing. BACE1 can be detected with different molecular weight: 42-56kD (prozymogen and isoforms), 65-75 (glycosylated forms) and 160kDa (dimer) (PMID:22741101; 21795680).

Publications

Species	Application	Title
human	WB	Small; Brain-Penetration and Neuron-Targeting DNA Nanoflowers Co-Delivering miR-124 and Rutin for Synergistic Therapy of Alzheimer's Disease.; Authors - Qin Ouyang
mouse	WB	Redox Biol; Hyperoside alleviates toxicity of β-amyloid via endoplasmic reticulum-mitochondrial calcium signal transduction cascade in APP/PS1 double transgenic Alzheimer's disease mice; Authors - Lin Lin Song
mouse	WB	Redox Biol; A potent phosphodiester Keap1-Nrf2 protein-protein interaction inhibitor as the efficient treatment of Alzheimer's disease; Authors - Yi Sun
mouse	WB	Proc Natl Acad Sci U S A; Aβ-induced Golgi fragmentation in Alzheimer's disease enhances Aβ production.; Authors - Gunjan Joshi
human,mouse	WB	Cell Rep; CIP2A Causes Tau/APP Phosphorylation, Synaptopathy, and Memory Deficits in Alzheimer's Disease.; Authors - Yang-Ping Shentu
mouse	WB	Stem Cell Reports; BACE1 Deficiency Causes Abnormal Neuronal Clustering in the Dentate Gyrus.; Authors - Hailong Hou ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xiaoyu (Verified Customer) (02-07-2024)

Good signal.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: brain

BACE1 Antibody Western Blot validation (1:1000 dilution) in brain (Cat no:12807-1-AP)