Anticorps Polyclonal de lapin anti-RHEB

• Phare
• Validé par KD/KO

RHEB Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (1)

N° de cat : 15924-1-AP

Synonymes

RHEB2, Ras homolog enriched in brain, GTP-binding protein Rheb, GTP binding protein Rheb, EC:3.6.5.-

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Galerie de données de validation

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  WB analysis using 15924-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 15924-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15924-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of 3T3-L1 using 15924-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 15924-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15924-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 15924-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15924-1-AP (RHEB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 15924-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15924-1-AP (RHEB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15924-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15924-1-AP (RHEB Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 15924-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15924-1-AP (RHEB Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human pancreas cancer using 15924-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (15924-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human pancreas cancer using 15924-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (15924-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse pancreas using 15924-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using RHEB antibody (15924-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 15924-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RHEB antibody (15924-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules 3T3-L1, cellules HeLa, tissu cérébral humain, tissu cérébral humain fœtal
• Résultats positifs en IHC: tissu cérébral humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du pancréas humain,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

15924-1-AP cible RHEB dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RHEB Protéine recombinante Ag8747
• Nom complet: Ras homolog enriched in brain
• Masse moléculaire calculée: 184 aa, 20 kDa
• Poids moléculaire observé: 15-20 kDa
• Numéro d’acquisition GenBank: BC016155
• Symbole du gène: RHEB
• Identification du gène (NCBI): 6009
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine/threonine kinase which augments protein synthesis. Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity.

Publications

Species	Application	Title
rat	WB	Cell Metab; Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle.; Authors - Noriaki Shimizu
mouse	IHC	PLoS Biol; RHEB/mTOR hyperactivity causes cortical malformations and epileptic seizures through increased axonal connectivity.; Authors - Martina Proietti Onori
mouse	IF	Metabolism; Rheb1 promotes glucose-stimulated insulin secretion in human and mouse β-cells by upregulating GLUT expression.; Authors - Yan Yang ; KO Validated
mouse	IF	iScience; Whitening of brown adipose tissue inhibits osteogenic differentiation via secretion of S100A8/A9; Authors - Ting Wang
human		Front Cell Dev Biol; PPT1 Reduction Contributes to Erianin-Induced Growth Inhibition in Oral Squamous Carcinoma Cells.; Authors - Qingqiong Luo
mouse	WB	Stem Cell Res Ther; Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis.; Authors - Caixia Jin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

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