TPT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TPT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 10824-1-AP

Synonyms

Translationally-controlled tumor protein, TCTP, p23, HRF, Histamine-releasing factor

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Validation Data Gallery

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  WB analysis of MCF-7 using 10824-1-AP

  WB result of TPT1 antibody (10824-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPT1 transfected MCF-7 cells.

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  WB analysis of mouse placenta using 10824-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 10824-1-AP (TPT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 10824-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10824-1-AP (TPT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 10824-1-AP

  IP result of anti-TPT1 (IP:10824-1-AP, 4ug; Detection:10824-1-AP 1:500) with human placenta tissue lysate 1520ug.

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  IHC staining of human colon cancer using 10824-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10824-1-AP (TPT1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 10824-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10824-1-AP (TPT1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10824-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10824-1-AP (TPT1 Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of HeLa using 10824-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TPT1 antibody (10824-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: A431 cells, mouse placenta tissue, MCF-7 cells
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

10824-1-AP targets TPT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TPT1 fusion protein Ag1268
• Full Name: tumor protein, translationally-controlled 1
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC003352
• Gene Symbol: TPT1
• Gene ID (NCBI): 7178
• RRID: AB_2287617
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein associated with cell growth and apoptosis regulation. It is overexpressed in most tumor cells and its down-regulation decreases the viability of those cells. This antibody detected 22-25 kDa TPT1 in lysates. An additional band around 50 kDa can also be observed occasionally, possibly representing the dimer form of TPT1.

Publications

Species	Application	Title
human	WB	Pharmacol Res; Tubocapsenolide A targets C-terminal cysteine residues of HSP90 to exert the anti-tumor effect.; Authors - Dongrong Zhu
human,mouse	WB,IHC,IF	Int J Biol Sci; Ailanthus Altissima-derived Ailanthone enhances Gastric Cancer Cell Apoptosis by Inducing the Repression of Base Excision Repair by Downregulating p23 Expression.; Authors - Chun-Ming Wang
human	WB	Chem Biol Interact; AMG900 as novel inhibitor of the translationally controlled tumor protein.; Authors - Nicolas Fischer
human	IP	Invest New Drugs; A novel ligand of the translationally controlled tumor protein (TCTP) identified by virtual drug screening for cancer differentiation therapy.; Authors - Nicolas Fischer
rat	WB,IHC	BMC Cancer; Identification of proteins involved in neural progenitor cell targeting of gliomas.; Authors - Staflin Karin K
human	WB	Cancer Sci; LncRNA TPT1-AS1 promotes tumorigenesis and metastasis in epithelial ovarian cancer by inducing TPT1 expression.; Authors - Weimin Wu ; KD Validated