RAF1 Polyclonal antibody

RAF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 26863-1-AP

Synonyms

Proto-oncogene c-RAF, Proto oncogene c RAF, NS5, EC:2.7.11.1, CRAF

---

Validation Data Gallery

• 

  WB analysis of DU 145 using 26863-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 26863-1-AP (RAF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 26863-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26863-1-AP (RAF1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26863-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26863-1-AP (RAF1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26863-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26863-1-AP (RAF1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: DU 145 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:600-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 13 publications below
• IF: See 1 publications below

Product Information

26863-1-AP targets RAF1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAF1 fusion protein Ag25423
• Full Name: v-raf-1 murine leukemia viral oncogene homolog 1
• Calculated Molecular Weight: 648 aa, 73 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC018119
• Gene Symbol: RAF1
• Gene ID (NCBI): 5894
• RRID: AB_2880660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Raf-1 proto-oncogene, serine/threonine kinase(RAF1), is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. RAF1 has two isoforms with MW of 73, 75 kDa. RAF1 plays an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

Publications

Species	Application	Title
human	WB	Oncogene; Targeting the p300/NONO axis sensitizes melanoma cells to BRAF inhibitors.; Authors - Feifei Zhang
human	WB	iScience; Intracellular magnetic hyperthermia reverses sorafenib resistance in hepatocellular carcinoma through its action on signaling pathways; Authors - Hugang Li
human	WB	Virol Sin; PLX8394, a RAF inhibitor, inhibits enterovirus 71 replication by blocking RAF/MEK/ERK signaling; Authors - Chengyuan Wu
human	WB	Mol Cell Probes; Elevated PGT promotes proliferation and inhibits cell apoptosis in preeclampsia by Erk signaling pathway; Authors - Huiyuan Pang
human	WB	FEBS Open Bio; CXCR4 promotes gefitinib resistance of Huh7 cells by activating the c-Met signaling pathway.; Authors - Dali Zhao
mouse	WB	Heliyon; Huanglian Jiedu decoction alleviates neurobehavioral damage in mice with chronic alcohol exposure through the RAS-RAF-MEK-ERK pathway; Authors - Yun Chen