Anticorps Polyclonal de lapin anti-RB1CC1

• Phare
• Validé par KD/KO

RB1CC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

95

Avis (1)

N° de cat : 17250-1-AP

Synonymes

RBICC, RB1-inducible coiled-coil protein 1, RB1CC 1, KIAA0203, FAK family kinase-interacting protein of 200 kDa

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 17250-1-AP

  WB result of RB1CC1 antibody (17250-1-AP, 1:2000) with si-Control and si-RB1CC1 transfected HEK293 cells.

• 

  WB analysis of HEK-293 using 17250-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17250-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17250-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17250-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17250-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17250-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 17250-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17250-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17250-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17250-1-AP (RB1CC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 17250-1-AP

  IP result of anti-RB1CC1 (IP:17250-1-AP, 5ug; Detection:17250-1-AP 1:500) with HEK-293 cells lysate 2500ug.

• 

  IP experiment of HEK-293 using 17250-1-AP

  IP result of anti-RB1CC1 (IP:17250-1-AP, 5ug; Detection:17250-1-AP 1:500) with HEK-293 cells lysate 1600ug.

• 

  IHC staining of mouse brain using 17250-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17250-1-AP (RB1CC1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17250-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17250-1-AP (RB1CC1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 17250-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17250-1-AP (RB1CC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 17250-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17250-1-AP (RB1CC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 17250-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17250-1-AP (RB1CC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 17250-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17250-1-AP (RB1CC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 17250-1-AP

  Immunofluorescent analysis of HepG2 cells, using RB1CC1 antibody 17250-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of MCF-7 using 17250-1-AP

  Immunofluorescent analysis of MCF-7 cells, using RB1CC1 antibody 17250-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat, cellules K-562, cellules MCF-7
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 16 publications below
• WB: See 65 publications below
• IHC: See 5 publications below
• IF: See 31 publications below
• IP: See 2 publications below
• CoIP: See 3 publications below

Informations sur le produit

17250-1-AP cible RB1CC1 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RB1CC1 Protéine recombinante Ag10508
• Nom complet: RB1-inducible coiled-coil 1
• Masse moléculaire calculée: 1594 aa, 183 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC017556
• Symbole du gène: RB1CC1
• Identification du gène (NCBI): 9821
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RB1CC1, also named as RBICC or FIP200, is implicated in the regulation of RB1 expression and functions as a DNA-binding transcription factor. It is a potent regulator of the RB1 pathway and a mediator that plays a crucial role in muscular differentiation. Its expression is, thus, a prerequisite for myogenic differentiation. Involved in autophagy. RB1CC1 is required for autophagosome formation. It is probably involved in the tumorigenesis of breast cancer. RB1CC1 is frequently mutated in breast cancer and shows characteristics of a classical tumor suppressor gene. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RB1CC1. the calculated molecular weight of RB1CC1 is 180 kDa, but the modified RB1CC1 is about 200 kDa.

Publications

Species	Application	Title
human	IF	Cell; Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes; Authors - Suresh Kumar
human	IF	Cell; CCT2 is an aggrephagy receptor for clearance of solid protein aggregates.; Authors - Xinyu Ma
mouse	WB	Nat Immunol; Essential role for GABARAP autophagy proteins in interferon-inducible GTPase-mediated host defense.; Authors - Miwa Sasai
human	WB,IF	Cell Res; A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.; Authors - Shulin Li ; KD Validated
human	WB	Nat Cell Biol; The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles.; Authors - Andrew M Leidal ; KO Validated
human	WB,IHC	Cancer Commun (Lond); Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies; Authors - Lu Tang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

SANGAPPA (Verified Customer) (01-04-2020)

Works very good in western blotting!

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Human endometrial stromal cells

Produits associés

RB1 Polyclonal antibody

10048-2-Ig

ULK1 Polyclonal antibody

20986-1-AP

mTOR Polyclonal antibody

20657-1-AP

ATG13 Polyclonal antibody

18258-1-AP

PIAS4 Polyclonal antibody

14242-1-AP

P53 Polyclonal antibody

10442-1-AP

PYK2 Polyclonal antibody

17592-1-AP