PYK2 Polyclonal antibody

PYK2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 17592-1-AP

Synonyms

CADTK, CAK beta, CAKB, Cell adhesion kinase beta, FADK 2, FADK2, FAK2, Focal adhesion kinase 2, FRNK, PKB, Proline rich tyrosine kinase 2, Protein tyrosine kinase 2 beta, PTK, PTK2B, PYK2, RAFTK

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Validation Data Gallery

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  WB analysis using 17592-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17592-1-AP (PYK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17592-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17592-1-AP (PYK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 17592-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17592-1-AP (PYK2 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17592-1-AP

  IP result of anti-PYK2 (IP:17592-1-AP, 4ug; Detection:17592-1-AP 1:500) with mouse brain tissue lysate 2640ug.

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  IHC staining of human kidney using 17592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17592-1-AP (PYK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17592-1-AP (PYK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 17592-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17592-1-AP (PYK2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17592-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17592-1-AP (PYK2 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells, rat brain tissue, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human kidney tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:3000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

The immunogen of 17592-1-AP is PYK2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PYK2 fusion protein Ag11487
• Full Name: PTK2B protein tyrosine kinase 2 beta
• Calculated Molecular Weight: 1009 aa, 116 kDa
• Observed Molecular Weight: 112-115 kDa
• GenBank Accession Number: BC042599
• Gene Symbol: PTK2B
• Gene ID (NCBI): 2185
• RRID: AB_10644483
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proline-rich tyrosine kinase 2 (Pyk2; also known as CAK, RAFTK and CADTK) is a cytoplasmic tyrosine kinase implicated to play a role in several intracellular signaling pathways. It is expressed in many cells and tissues and migrated as a 130 kDa band in Western Blotting analysis. The size of PYK2 is 115 kDa, and it expressed in many cells and tissues and migrated as a 130 kDa band, but several lower molecular weight bands (75 kDa, 80 kDa, and 97 kDa) were seen in Western Blotting analysis, possibly due to proteolytic degradation.

Publications

Species	Application	Title
mouse	WB	Int J Cancer; Plant galactolipid dLGG suppresses lung metastasis of melanoma through deregulating TNF-α-mediated pulmonary vascular permeability and circulating oxylipin dynamics in mice.; Authors - Chung-Chih Yang
mouse	WB	Mucosal Immunol; ADP/P2Y1 aggravates inflammatory bowel disease through ERK5-mediated NLRP3 inflammasome activation.; Authors - Chengfei Zhang
human,mouse	IHC	EBioMedicine; Biallelic mutations in LAMA5 disrupts a skeletal noncanonical focal adhesion pathway and produces a distinct bent bone dysplasia.; Authors - Maya Barad
human	WB	Cell Signal; Identification of NCK1 as a novel downstream effector of STAT3 in colorectal cancer metastasis and angiogenesis.; Authors - Fan Zhang
human	WB	Stem Cell Rev Rep; A Novel Splicing Mutation c.335-1 G > A in the Cardiac Transcription Factor NKX2-5 Leads to Familial Atrial Septal Defect Through miR-19 and PYK2.; Authors - Li Jia
human	WB,IP	Cell Chem Biol; Differential network analysis of ROS1 inhibitors reveals lorlatinib polypharmacology through co-targeting PYK2; Authors - Yi Liao