PIAS4 Polyclonal antibody

• Featured Product
• KD/KO Validated

PIAS4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 14242-1-AP

Synonyms

E3 SUMO protein ligase PIAS4, PIAS gamma, PIAS4, Piasg, PIASY, ZMIZ6

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Validation Data Gallery

• 

  WB analysis of K-562 using 14242-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14242-1-AP (PIAS4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 14242-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14242-1-AP (PIAS4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 14242-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 14242-1-AP (PIAS4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 14242-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14242-1-AP (PIAS4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: K-562 cells, HL-60 cells, mouse ovary tissue, mouse pancreas tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IF: See 3 publications below

Product Information

14242-1-AP targets PIAS4 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PIAS4 fusion protein Ag5498
• Full Name: protein inhibitor of activated STAT, 4
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57-70 kDa
• GenBank Accession Number: BC029874
• Gene Symbol: PIAS4
• Gene ID (NCBI): 51588
• RRID: AB_2164365
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PIAS4(protein inhibitor of activated STAT protein 4) is also named as PIASG, PIASy and belongs to the PIAS family. It enhanced the conjugation of SUMO2 to GATA2 through its E3 SUMO ligase activity. TGF-b can up-regulates PLAS4 expression in MCs to down-regulatea-SMA gene transcription by the interaction with E12 and PLAS4 predominantly acts as E3 ligases for E12 in MCs among PIAS family members (PMID:22829926). This protein can be sumoylated and Lys-35 is the main site of sumoylation(PMID:15831457).

Publications

Species	Application	Title
human	WB,IF	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
human	WB	Nat Commun; Rad18-dependent SUMOylation of human specialized DNA polymerase eta is required to prevent under-replicated DNA.; Authors - Emmanuelle Despras ; KD Validated
mouse	WB,IF	PLoS Biol; DPPA2/4 and SUMO E3 ligase PIAS4 opposingly regulate zygotic transcriptional program.; Authors - Yao-Long Yan ; KD Validated
mouse	WB	PLoS One; Protein inhibitor of activated STAT, PIASy regulates α-smooth muscle actin expression by interacting with E12 in mesangial cells.; Authors - Torikoshi Kazuo K ; KD Validated
human	WB	Front Nutr; Hengshun Aromatic Vinegar Ameliorates Vascular Endothelial Injury via Regulating PKCζ-Mediated Oxidative Stress and Apoptosis.; Authors - Xin Li
human	WB	Nat Commun; Suppression of ACE2 SUMOylation protects against SARS-CoV-2 infection through TOLLIP-mediated selective autophagy; Authors - Shouheng Jin ; KD Validated