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  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-PPAR Gamma

PPAR Gamma Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 22061-1-AP

Synonymes

CIMT1, NR1C3, PPAR gamma, PPARG, PPARG2, PPARγ



Applications testées

Résultats positifs en WBcellules HEK-293, cellules 3T3-L1, cellules HL-60, cellules MCF-7, tissu cardiaque de souris, tissu cardiaque humain, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu hépatique de rat, tissu hépatique de souris, tissu ovarien de souris, tissu testiculaire de souris
Résultats positifs en IHCtissu de cancer de la prostate humain, tissu de cancer du côlon humain, tissu de côlon de rat, tissu placentaire humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochimie (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

22061-1-AP cible PPAR Gamma dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène PPAR Gamma Protéine recombinante Ag17136
Nom complet peroxisome proliferator-activated receptor gamma
Masse moléculaire calculée 505 aa, 58 kDa
Poids moléculaire observé 66-70 kDa
Numéro d’acquisition GenBankBC006811
Symbole du gène PPARG
Identification du gène (NCBI) 5468
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Peroxisome Proliferator-Activated Receptors (PPARs) are ligand-activated intracellular transcription factors, members of the nuclear hormone receptor superfamily (NR), that includes estrogen, thyroid hormone receptors, retinoic acid, Vitamin D3 as well as retinoid X receptors (RXRs). The PPAR subfamily consists of three subtypes encoded by distinct genes denoted PPARα (NR1C1), PPARβ/δ (NR1C2) and PPARγ (NR1C3), which are activated by selective ligands. PPARγ, also named as PPARG, contains one nuclear receptor DNA-binding domain and is a receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. It plays an important role in the regulation of lipid homeostasis, adipogenesis, INS resistance, and development of various organs. Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) and may be associated with susceptibility to obesity. Defects in PPARG can lead to type 2 INS-resistant diabetes and hypertension. PPARG mutations may be associated with colon cancer. Genetic variations in PPARG are associated with susceptibility to glioma type 1 (GLM1). PPARG has two isoforms with molecular weight 57 kDa and 54 kDa (PMID: 9831621), but modified PPARG is about 67 KDa (PMID: 16809887). PPARG2 is a splice variant and has an additional 30 amino acids at the N-terminus (PMID: 15689403). Experimental data indicate that a 45 kDa protein displaying three different sequences immunologically related to the nuclear receptor PPARG2 is located in mitochondria (mt-PPAR). However, the molecular weight of this protein is clearly less when compared to that of PPARG2 (57 kDa). (PMID: 10922459). PPARG has been reported to be localized mainly (but not always) in the nucleus. PPARG can also be detected in the cytoplasm and was reported to possess extra-nuclear/non-genomic actions (PMID: 17611413; 19432669; 14681322).

Protocole

Product Specific Protocols
WB protocol for PPAR Gamma antibody 22061-1-APDownload protocol
IHC protocol for PPAR Gamma antibody 22061-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Autophagy

Mir223 restrains autophagy and promotes CNS inflammation by targeting ATG16L1.

Authors - Yan Li
mouseWB

Genes Dev

Loss of TLE3 promotes the mitochondrial program in beige adipocytes and improves glucose metabolism.

Authors - Stephanie Pearson
mouseWB

Free Radic Biol Med

Hydrogen sulfide alleviates particulate matter-induced emphysema and airway inflammation by suppressing ferroptosis.

Authors - Ying Wang
mouseWB

Biochem Pharmacol

A novel pyrazole-containing indolizine derivative suppresses NF-κB activation and protects against TNBS-induced colitis via a PPAR-γ-dependent pathway.

Authors - Yong Fu

Front Pharmacol

The Active Components of Fuzheng Huayu Formula and Their Potential Mechanism of Action in Inhibiting the Hepatic Stellate Cells Viability - A Network Pharmacology and Transcriptomics Approach.

Authors - Xinrui Xing
ratWB

Front Pharmacol

Alpha-Asaronol Alleviates Dysmyelination by Enhancing Glutamate Transport Through the Activation of PPARγ-GLT-1 Signaling in Hypoxia-Ischemia Neonatal Rats.

Authors - Yuhang Ge