Adiponectin Monoclonal antibody

Adiponectin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

5D8A7

Cat no : 66239-1-Ig

Synonyms

ACDC, ACRP30, adiponectin, ADIPOQ, ADIPQTL1, ADPN, APM 1, APM1, GBP28, Gelatin binding protein

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Validation Data Gallery

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  WB analysis of human adipose using 66239-1-Ig

  human adipose tissue were subjected to SDS PAGE followed by western blot with 66239-1-Ig (ADIPOQ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 66239-1-Ig (ADIPOQ antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 66239-1-Ig (ADIPOQ antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brown adipose using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brown adipose using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brown adipose slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brown adipose using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brown adipose slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 66239-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66239-1-Ig (Adiponectin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of 3T3-L1 using 66239-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed 3T3-L1 cells using Adiponectin antibody (66239-1-Ig, Clone: 5D8A7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: human adipose tissue
• Positive IHC detected in: mouse skeletal muscle tissue, mouse skin tissue, human placenta tissue, human prostate cancer tissue, mouse brown adipose tissue, rat brown adipose tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: 3T3-L1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:16000-1:64000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 1 publications below

Product Information

66239-1-Ig targets Adiponectin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Adiponectin fusion protein Ag17383
• Full Name: adiponectin, C1Q and collagen domain containing
• Calculated Molecular Weight: 244 aa, 26 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC096308
• Gene Symbol: Adiponectin
• Gene ID (NCBI): 9370
• RRID: AB_2881628
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.

Publications

Species	Application	Title
mouse	WB	J Cell Physiol; Rapid responses of adipocytes to iron overload increase serum TG level by decreasing adiponectin.; Authors - Yuxiao Tang
mouse	WB	Food Funct; Quercetin regulates skeletal muscle fiber type switching via adiponectin signaling.; Authors - Xiaoling Chen
pig	WB	Anim Biotechnol; Effect of dietary leucine supplementation on skeletal muscle fiber type transformation in weaning piglets; Authors - Xiaoling Chen
pig	WB	Anim Biotechnol; Leucine regulates porcine muscle fiber type transformation via adiponectin signaling pathway.; Authors - Lu Xiang
human	WB,IF	Chem Biol Interact; Inhibitory effects of black phosphorus nanosheets on tumor cell proliferation through downregulation of ADIPOQ and downstream signaling pathways; Authors - Daxu Liu
mouse	WB	Signal Transduct Target Ther; Annexin A1 binds PDZ and LIM domain 7 to inhibit adipogenesis and prevent obesity; Authors - Lu Fang