PPARA Polyclonal antibody

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  WB analysis using 15540-1-AP

  C2C12 cells were subjected to SDS PAGE followed by western blot with 15540-1-AP (PPARA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of U-937 using 15540-1-AP

  IP result of anti-PPARA (IP:15540-1-AP, 4ug; Detection:15540-1-AP 1:300) with U-937 cells lysate 4000ug.

• Featured Product
• KD/KO Validated

PPARA Polyclonal Antibody for WB, IP, ELISA

Cat No. 15540-1-AP

Publications(190)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IF, IP, CoIP, ChIP, ELISA

Synonyms

hPPAR, NR1C1, PPAR, PPAR alpha, PPARA, PPARα

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Tested Applications

• Positive WB detected in: C2C12 cells
• Positive IP detected in: U-937 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 13 publications below
• WB: See 180 publications below
• IF: See 13 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Product Information

15540-1-AP targets PPARA in WB, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken, bovine, hamster, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PPARA fusion protein Ag7896
• Full Name: peroxisome proliferator-activated receptor alpha
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC000052
• Gene Symbol: PPARA
• Gene ID (NCBI): 5465
• RRID: AB_2252506
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q07869
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peroxisome proliferator-activated receptor alpha (PPARA) is a ligand-activated transcription factor that belongs to the PPAR nuclear receptor superfamily. PPARA is essential in the modulation of lipid transport and metabolism, mainly through activating mitochondrial and peroxisomal fatty acid β-oxidation pathways. In addition, PPARA seems to decrease inflammation mainly through direct interaction with NF-κB, causing inhibition of its signaling pathway or reducing the activated levels of NF-κB and subsequent inflammation. Furthermore, PPARA was implicated in the attenuation of oxidative stress in alcoholic liver disease when treated with polyenephosphatidylcholine through downregulation of ROS-generating enzymes such as ethanol-inducible cytochrome P450 2E1 (CYP2E1), acyl-CoA oxidase, and NADPH oxidase. PPARA exists two isoforms and molecular weight of PPARA isoforms are 52 kDa and 22 kDa. The ability of a retinoid X receptor (RXR) to heterodimerize with many nuclear receptors, including LXR, PPAR, NGF1B and RAR, underscores its pivotal role within the nuclear receptor superfamily. Among these heterodimers, PPAR:RXR is considered an important signalling mediator of both PPAR ligands, such as fatty acids, and 9-cis retinoic acid (9-cis RA), an RXR ligand. (PMID: 15103326 ). PPARA can form Heterodimer with RXRA and molecular weight of Heterodimer is about 110 kDa.

Publications

Species	Application	Title
mouse	IF	Nat Commun; Hyodeoxycholic acid ameliorates nonalcoholic fatty liver disease by inhibiting RAN-mediated PPARα nucleus-cytoplasm shuttling; Authors - Jing Zhong
human	WB	Sci Adv; Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment.; Authors - Leiming Wang
mouse	WB	Hepatology; The histone methyltransferase Suv39h2 contributes to nonalcoholic steatohepatitis in mice.; Authors - Zhiwen Fan
mouse	WB	Nat Commun; Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.; Authors - Fangjun Yu
human	WB	Nat Commun; N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.; Authors - Yanying Wang
mouse	WB	Nat Commun; Hepatic HuR modulates lipid homeostasis in response to high-fat diet.; Authors - Zhuojun Zhang