RXRA Polyclonal antibody

• Featured Product
• KD/KO Validated

RXRA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

38

Cat no : 21218-1-AP

Synonyms

ORF-3, U66306, RXRα, Retinoic acid receptor RXR-alpha, ORF3

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Validation Data Gallery

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  WB analysis using 21218-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21218-1-AP (RXRA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 21218-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21218-1-AP (RXRA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HCT 116 using 21218-1-AP

  HCT 116 lysates prepared and IP of RXRA performed using 1.0 μg of 21218-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IP experiment of MCF-7 using 21218-1-AP

  IP result of anti-RXRA (IP:21218-1-AP, 4ug; Detection:21218-1-AP 1:600) with MCF-7 cells lysate 800ug.

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  IHC staining of human kidney using 21218-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21218-1-AP (RXRA antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach cancer using 21218-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21218-1-AP (RXRA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 21218-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21218-1-AP (RXRA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 21218-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21218-1-AP (RXRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 21218-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21218-1-AP (RXRA antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 21218-1-AP

  Immunofluorescent analysis of Hela cells, using RXRA antibody 21218-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, MCF-7 cells
• Positive IP detected in: MCF-7 cells, HCT 116 cells
• Positive IHC detected in: human kidney tissue, mouse heart tissue, human stomach cancer tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 24 publications below
• IHC: See 4 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• ChIP: See 5 publications below
• RIP: See 1 publications below

Product Information

21218-1-AP targets RXRA in WB, IHC, IF/ICC, IP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, duck
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RXRA fusion protein Ag15651
• Full Name: retinoid X receptor, alpha
• Calculated Molecular Weight: 462 aa, 51 kDa
• Observed Molecular Weight: 50-54 kDa
• GenBank Accession Number: BC110998
• Gene Symbol: RXRA
• Gene ID (NCBI): 6256
• RRID: AB_10693633
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Retinoid X receptor alpha (RXRA). Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high-affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of a ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation, and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. This antibody is a rabbit polyclonal antibody raised against the 350 AA of human RXRA C-terminal. RXRA is highly expressed in the liver and also expressed in the lungs, kidneys, and heart. It can recognize the mature 54 kDa RXRA and the truncated 44 kDa RXRA (PMID: 20541701).

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB	Nat Genet; Comprehensive characterization of protein-protein interactions perturbed by disease mutations.; Authors - Feixiong Cheng
human	WB	Sci Adv; Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment.; Authors - Leiming Wang
mouse	WB	Redox Biol; Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke; Authors - Haiyun Guo
human	ChIP	Lab Anim (NY); Animal models of male subfertility targeted on LanCL1-regulated spermatogenic redox homeostasis.; Authors - Chao Huang
human	WB	BMC Med; Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis; Authors - Zhijian Zheng