Anticorps Monoclonal anti-CD31

CD31 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

26

CloneNo.

2A1E2

N° de cat : 66065-1-Ig

Synonymes

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Galerie de données de validation

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  WB analysis of Jurkat using 66065-1-Ig

  Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 66065-1-Ig (CD31 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

• 

  WB analysis using 66065-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66065-1-Ig (CD31 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66065-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66065-1-Ig (PECAM1,CD31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 66065-1-Ig

  Untreated and PNGase F-treated lysates of THP-1 cells were subjected to SDS PAGE followed by western blot with 66065-1-Ig (CD31 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

• 

  IHC staining of human liver cancer using 66065-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-1-Ig (CD31 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66065-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66065-1-Ig (CD31 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66065-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66065-1-Ig (CD31 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66065-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66065-1-Ig (CD31 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66065-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-1-Ig (CD31 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66065-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66065-1-Ig (CD31 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 66065-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed human tonsillitis tissue using 66065-1-Ig(CD31 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 66065-1-Ig

  Immunofluorescent analysis of (4% PFA ) fixed human tonsillitis tissue using 66065-1-Ig(CD31 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules THP-1, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu d'amygdalite humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'amygdalite humain

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:8000
• Immunohistochimie (IHC): IHC : 1:400-1:4000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 5 publications below
• IF: See 11 publications below

Informations sur le produit

66065-1-Ig cible CD31 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD31 Protéine recombinante Ag1787
• Nom complet: platelet/endothelial cell adhesion molecule
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: BC022512
• Symbole du gène: PECAM1
• Identification du gène (NCBI): 5175
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Publications

Species	Application	Title
human	IF	Nature; Construction of a human cell landscape at single-cell level.; Authors - Xiaoping Han
		Biomaterials; An iRGD-conjugated prodrug micelle with blood-brain-barrier penetrability for anti-glioma therapy.; Authors - Lu Lu
human	IF	Small; Airflow-Assisted 3D Bioprinting of Human Heterogeneous Microspheroidal Organoids with Microfluidic Nozzle.; Authors - Haiming Zhao
human	IF	Theranostics; Single-cell landscape reveals active cell subtypes and their interaction in the tumor microenvironment of gastric cancer.; Authors - Yumin Li
	IF	Theranostics; Encapsulation of LXR ligand by D-Nap-GFFY hydrogel enhances anti-tumorigenic actions of LXR and removes LXR-induced lipogenesis.; Authors - Ke Feng
	IHC	Cancer Lett; CD44(+) tumor cells promote early angiogenesis in head and neck squamous cell carcinoma.; Authors - Nils Ludwig

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