E-selectin / CD62E Polyclonal antibody

E-selectin / CD62E Polyclonal Antibody for FC, IHC, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 20894-1-AP


CD62E, ELAM, ELAM 1, ELAM1, ESEL, E-selectin, E-selectin /CD62E, LECAM2, SELE, selectin E

Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHUVEC cells

Recommended dilution

Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

20894-1-AP targets E-selectin / CD62E in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen E-selectin / CD62E fusion protein Ag14944
Full Name selectin E
Calculated molecular weight 610 aa, 67 kDa
GenBank accession numberBC142711
Gene symbol SELE
Gene ID (NCBI) 6401
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

E-selectin, also known as ELAM-1 or CD62E, is a member of the selectin family of adhesion molecules that also includes L-selectin and P-selectin. E-selectin is an inducible endothelial cell surface molecule. Its expression on endothelial cells is transcriptionally upregulated by various proinflammatory substances such as IL-1, TNFα and lipopolysaccharide (LPS). Besides, it can be secreted by endothelial cells, and can be detected in a soluble form (sE-selectin) in serum. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. (PMID: 2827173; 1382417; 8943958)


Product Specific Protocols
IHC protocol for E-selectin / CD62E antibody 20894-1-APDownload protocol
FC protocol for E-selectin / CD62E antibody 20894-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Adv Sci (Weinh)

Astrocyte-Derived Extracellular Vesicular miR-143-3p Dampens Autophagic Degradation of Endothelial Adhesion Molecules and Promotes Neutrophil Transendothelial Migration after Acute Brain Injury

Authors - Xun Wu

Sci Adv

The support of genetic evidence for cardiovascular risk induced by antineoplastic drugs.

Authors - Hui Cui

Biomater Sci

An E-selectin targeting and MMP-2-responsive dextran-curcumin polymeric prodrug for targeted therapy of acute kidney injury

Authors - Jing-Bo Hu


MIN score predicts primary response to infliximab/adalimumab and vedolizumab therapy in patients with inflammatory bowel diseases.

Authors - Yuan Shi

Int J Mol Sci

Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.

Authors - Federica Morani

J Neuroinflammation

Downregulation of CD151 restricts VCAM-1 mediated leukocyte infiltration to reduce neurobiological injuries after experimental stroke.

Authors - Ceshu Gao


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Yannic (Verified Customer) (01-07-2022)

Left and right band refer to markers. Many unspecific bands, blocked with 5% BSA, primary antibody over night

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HMEC1 endothelial cells
E-selectin / CD62E Antibody Western Blot validation (1:1000 dilution) in HMEC1 endothelial cells (Cat no:20894-1-AP)