CD38 Monoclonal antibody

• Featured Product
• KD/KO Validated

CD38 Monoclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

3C6G4

Reviews (1)

Cat no : 60006-1-Ig

Synonyms

ADP ribosyl cyclase 1, cADPr hydrolase 1, CD38, CD38 molecule, Cyclic ADP ribose hydrolase 1, T10

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Validation Data Gallery

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  WB analysis of Jurkat using 60006-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60006-1-Ig (CD38 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 60006-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60006-1-Ig (CD38 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 60006-1-Ig

  Immunofluorescent analysis of NIH/3T3 cells using 60006-1-Ig (CD38 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

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  FC experiment of Daudi using 60006-1-Ig

  1X10^6 Daudi cells were intracellularly stained with 0.4 ug Anti-Human CD38 (60006-1-Ig, Clone:3C6G4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Jurkat cells, Raji cells
• Positive IF detected in: NIH/3T3 cells
• Positive FC detected in: Daudi cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

60006-1-Ig targets CD38 in WB, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD38 fusion protein Ag0487
• Full Name: CD38 molecule
• Calculated molecular weight: 300 aa, 34 kDa
• Observed molecular weight: 45 kDa
• GenBank accession number: BC007964
• Gene symbol: CD38
• Gene ID (NCBI): 952
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).

Publications

Species	Application	Title
human	WB	Nat Immunol; PD-1 blockade in subprimed CD8 cells induces dysfunctional PD-1+CD38hi cells and anti-PD-1 resistance.; Authors - Vivek Verma ; KD Validated
mouse	WB	Glia; Activation of astrocytic sigma-1 receptor exerts antidepressant-like effect via facilitating CD38-driven mitochondria transfer.; Authors - Yun Wang ; KD Validated
human	IHC	Cancer Cell Int; Targeting Lymphotoxin Beta and Paired Box 5: a potential therapeutic strategy for soft tissue sarcoma metastasis.; Authors - Runzhi Huang
	IF	Cell Rep; Longitudinal analyses reveal distinct immune response landscapes in lung and intestinal tissues from SARS-CoV-2-infected rhesus macaques.; Authors - Huiwen Zheng
human		J Immunol; Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues; Authors - Teresa W-M Fan
mouse	WB	J Nutr Biochem; Both prolonged high-fat diet consumption and calorie restriction boost hepatic NAD+ metabolism in mice; Authors - Xiaojing Wei

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Komuraiah (Verified Customer) (07-16-2021)

CD38 expression that I tested in kidney tissue from control and cisplatin induced kidney injury model. This antibody is working great with high specificity with suggested molecular size.

• Applications: Western Blot

CD38 Antibody Western Blot validation ( dilution) in (Cat no:60006-1-Ig)