CD38 Monoclonal antibody

• Featured Product
• KD/KO Validated

CD38 Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, IF-P, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

13

CloneNo.

3C6G4

Reviews (1)

Cat no : 60006-1-Ig

Synonyms

ADPRC 1, ADP ribosyl cyclase 1, 3C6G4, 2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase

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Validation Data Gallery

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  WB analysis using 60006-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60006-1-Ig (CD38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 60006-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60006-1-Ig (CD38 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse spleen using 60006-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD38 antibody (60006-1-Ig, Clone: 3C6G4 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 60006-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD38 antibody (60006-1-Ig, Clone: 3C6G4 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Raji using 60006-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD38 antibody (60006-1-Ig, Clone: 3C6G4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

• Positive WB detected in: A549 cells, Daudi cells, Jurkat cells, NK-92 cells, THP-1 cells, Ramos cells, Raji cells, TF-1 cells
• Positive IF-P detected in: mouse spleen tissue
• Positive IF/ICC detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 3 publications below

Product Information

60006-1-Ig targets CD38 in WB, IF/ICC, IF-P, ELISA, Cell treatment applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD38 fusion protein Ag0487
• Full Name: CD38 molecule
• Calculated Molecular Weight: 300 aa, 34 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC007964
• Gene Symbol: CD38
• Gene ID (NCBI): 952
• RRID: AB_716994
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).

Publications

Species	Application	Title
human	WB	Nat Immunol; PD-1 blockade in subprimed CD8 cells induces dysfunctional PD-1+CD38hi cells and anti-PD-1 resistance.; Authors - Vivek Verma ; KD Validated
human	WB	Pharmacol Res; Targeting CD38/ ADP-ribosyl cyclase as a novel therapeutic strategy for identification of three potent agonists for leukopenia treatment; Authors - Yuanzhi Liu
mouse	WB	Glia; Activation of astrocytic sigma-1 receptor exerts antidepressant-like effect via facilitating CD38-driven mitochondria transfer.; Authors - Yun Wang ; KD Validated
human		Cancer Cell Int; Targeting Lymphotoxin Beta and Paired Box 5: a potential therapeutic strategy for soft tissue sarcoma metastasis.; Authors - Runzhi Huang
	IF	Cell Rep; Longitudinal analyses reveal distinct immune response landscapes in lung and intestinal tissues from SARS-CoV-2-infected rhesus macaques.; Authors - Huiwen Zheng
human	WB	NPJ Aging; The use of a systems approach to increase NAD+ in human participants; Authors - John D Henderson

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Komuraiah (Verified Customer) (07-16-2021)

CD38 expression that I tested in kidney tissue from control and cisplatin induced kidney injury model. This antibody is working great with high specificity with suggested molecular size.

• Applications: Western Blot

CD38 Antibody Western Blot validation ( dilution) in (Cat no:60006-1-Ig)