- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-PAX6
PAX6 Polyclonal Antibody for WB, IF, IHC, ELISA, FC (Intra)
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (1)
Applications
WB, IF, IHC, ELISA, FC (Intra)
Conjugaison
Non conjugué
N° de cat : 12323-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules Y79, tissu cérébral de rat, tissu d'estomac humain, tissu embryonnaire de souris |
| Résultats positifs en IHC | tissu cérébral de souris, tissu de rétinoblastome humain, tissu embryonnaire de souris, tissu oculaire de souris il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF | Retinal organoids, cellules iPS |
| Résultats positifs en FC (Intra) | cellules SH-SY5Y |
| Résultats positifs en cytométrie | cellules SH-SY5Y |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochimie (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 1 publications below |
| WB | See 14 publications below |
| IHC | See 6 publications below |
| IF | See 42 publications below |
Informations sur le produit
12323-1-AP cible PAX6 dans les applications de WB, IF, IHC, ELISA, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris
| Réactivité | Humain, rat, souris |
| Réactivité citée | rat, Humain, singe, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | PAX6 Protéine recombinante Ag2984 |
| Nom complet | paired box 6 |
| Masse moléculaire calculée | 47 kDa |
| Poids moléculaire observé | 47 kDa |
| Numéro d’acquisition GenBank | BC011953 |
| Symbole du gène | PAX6 |
| Identification du gène (NCBI) | 5080 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Background
Paired-box 6 (Pax6) is a highly conserved nuclear transcription factor that is key in neural tissue development, particularly eye morphogenesis.
What is the molecular weight of Pax6?
46.6 kDa. Pax6 is composed of 422 amino acids and contains a paired domain and a homeodomain.
What is the role of Pax6 in development?
In embryos, Pax6 is expressed in the developing central nervous system (CNS), eye, nose, and also in the pancreas. As a transcription factor, Pax6 is one of the earliest genes expressed in the eye and regulates the expression of many genes required for retinal and olfactory development (PMID: 10461206). The broad expression of Pax6 in the neuroectoderm, which develops to the CNS, makes it a useful marker for neural stem cells (PMID: 20621053).
The homology of Pax6 to the Drosophila gene eyeless and the mouse gene Small eye highlights its importance across a wide spectrum of organisms. In model organisms such as flies or mice, a dramatic phenotype can be seen. Mutations in eyeless lead to ectopic eye development and mice with heterozygote small eye mutations do not develop eyes or nasal cavities, and homozygote small eye mice do not survive (PMID: 7892602; PMID 8875254).
What is the role of Pax6 in eye disease?
Due to its key role as a master regulator in eye development, mutations in Pax6 have been associated with many different eye diseases. In humans, premature stop signals that lead to the production of a truncated protein have been found to cause aniridia, the absence of an iris (PMID: 19898691). Single amino acid mutations in Pax6 may lead to Peters' anomaly, which causes thinning and clouding of the cornea (PMID: 8162071).
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for PAX6 antibody 12323-1-AP | Download protocol |
| IHC protocol for PAX6 antibody 12323-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis. | ||
Cell Stem Cell Chemical-induced chromatin remodeling reprograms mouse ESCs to totipotent-like stem cells. | ||
Cell Metab Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells. | ||
Cell Rep Med End binding-3 inhibitor activates regenerative program in age-related macular degeneration | ||
Cell Rep Astrocytes mediate cell non-autonomous correction of aberrant firing in human FXS neurons |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ryszard (Verified Customer) (01-19-2024) | Antibody Pax-6 Rabbit works very well on whole mount mESC-derived cerebral organoids.
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FH Julie (Verified Customer) (01-18-2021) | This antibody works very well for immunofluorescence on human brain.
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FH Rachel (Verified Customer) (02-01-2019) | Worked well and Proteintech employees were very helpful during the ordering process.
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