MAF Polyclonal antibody

MAF Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 55013-1-AP

Synonyms

c MAF, cMAF, MAF, Proto oncogene c Maf, Transcription factor Maf



Tested Applications

Positive WB detected inHeLa cells, A431 cells, K-562 cells, A375 cells, HepG2 cells
Positive IP detected inA431 cells
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

55013-1-AP targets MAF in WB, IP, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian)
Calculated molecular weight 42 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberNM_005360
Gene symbol MAF
Gene ID (NCBI) 4094
RRIDAB_10863127
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAF, also named as c-Maf, belongs to the bZIP family and Maf subfamily. MAF acts as a transcriptional activator or repressor. It is involved in embryonic lens fiber cell development. MAF increases T cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, it transactivates strongly the glucagon gene promoter through the G1 element. MAF activates transcription of the CD13 proximal promoter in endothelial cells. It is involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. When overexpressed, MAF represses anti-oxidant reponse element (ARE)-mediated transcription. It is involved either as an oncogene or as a tumor suppressor, depending on the cell context. A chromosomal aberration involving MAF is found in some forms of multiple myeloma (MM). Defects in MAF are the cause of cataract pulverulent juvenile-onset MAF-related (CAPJOM). Defects in MAF are the cause of cataract congenital cerulean type 4 (CCA4). The antibody is specific to MAF. And it could recognise the 50 kDa band that also be detected in the paper (PMID: 25770584 ) .

Protocols

Product Specific Protocols
WB protocol for MAF antibody 55013-1-APDownload protocol
IF protocol for MAF antibody 55013-1-APDownload protocol
IP protocol for MAF antibody 55013-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Immunity

RAS P21 Protein Activator 3 (RASA3) Specifically Promotes Pathogenic T Helper 17 Cell Generation by Repressing T-Helper-2-Cell-Biased Programs.

Authors - Bing Wu
humanWB

Acta Pharmacol Sin

Mebendazole elicits potent antimyeloma activity by inhibiting the USP5/c-Maf axis.

Authors - Xue-Han Chen
mouseWB

Int J Mol Sci

Deletion of Cdk5 in Macrophages Ameliorates Anti-Inflammatory Response during Endotoxemia through Induction of C-Maf and Il-10

Authors - Pauline Pfänder
humanWB

Front Immunol

Molecular Mechanisms Driving IL-10- Producing B Cells Functions: STAT3 and c-MAF as Underestimated Central Key Regulators?

Authors - Magalie Michée-Cospolite
mouseWB

J Immunol

Malat1 Suppresses Immunity to Infection through Promoting Expression of Maf and IL-10 in Th Cells.

Authors - James P Hewitson
humanWB

Cell Commun Signal

Inhibition of the Otub1/c-Maf axis by the herbal acevaltrate induces myeloma cell apoptosis.

Authors - Tong Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Katie (Verified Customer) (04-08-2022)

Works well for western blot. We have published with this antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Primary CD4 T cells
FH

H (Verified Customer) (04-05-2020)

This protein has a very short half life, and present in the nucleus. I could see a clear band after the nuclear-cytosolic fractionation.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
MAF Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:55013-1-AP)