OCT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

OCT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 10387-1-AP

Synonyms

OTF1, Octamer-binding transcription factor 1, Octamer-binding protein 1, Oct-1, NF-A1

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Validation Data Gallery

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  WB analysis using 10387-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10387-1-AP (OCT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT using 10387-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 10387-1-AP (OCT1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10387-1-AP

  IP result of anti-OCT1 (IP:10387-1-AP, 4ug; Detection:10387-1-AP 1:300) with HeLa cells lysate 1600ug.

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  IHC staining of human colon cancer using 10387-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10387-1-AP (OCT1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10387-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10387-1-AP (OCT1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 10387-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10387-1-AP (OCT1 antibody) at dilution of 1:100 (under 25x lens).

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  IF Staining of HeLa using 10387-1-AP

  Immunofluorescent analysis of HeLa cells using 10387-1-AP (OCT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10387-1-AP

  Immunofluorescent analysis of HeLa cells using 10387-1-AP (OCT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, NCCIT cells, HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 2 publications below

Product Information

10387-1-AP targets OCT1 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OCT1 fusion protein Ag0586
• Full Name: POU class 2 homeobox 1
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC003571
• Gene Symbol: POU2F1
• Gene ID (NCBI): 5451
• RRID: AB_2167052
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The POU class 2 homeobox 1 (POU2F1), also known as octamer-binding transcription factor-1 (OCT-1), is a ubiquitous nuclear protein that regulates the transcription of target genes by binding to a cis-acting octamer element(5'-ATTTGCAT-3'). It participated in the regulation of housekeeping genes, including histone H2B and snRNAs, for cell differentiation and proliferation [PMID:3462701]. In addition, it functions as a cellular stress sensor that modulates activities of tissue-specific target genes involved in immune and inflammatory responses [PMID:16322220]. It also acts as a repressor for the cytokine-inducible, NF-kB-dependent expression of E-selectin and the vascular cell adhesion molecule during vascular inflammation. Otherwise, it can interact with nuclear hormone receptors, such as the retinoid X receptor, the thyroid hormone receptor and the glucocorticoid receptor, and influence their transcription activity[PMID:17192276].

Publications

Species	Application	Title
pig	ChIP	Mol Biol Evol; Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells.; Authors - Ke Chen
mouse	IF	Food Funct; Anti-hyperuricemic potential of stevia (Stevia rebaudiana Bertoni) residue extract in hyperuricemic mice; Authors - Arshad Mehmood
rat	WB	Food Funct; Ferulic acid supplementation alleviates hyperuricemia in high-fructose/fat diet-fed rats via promoting uric acid excretion and mediating the gut microbiota; Authors - Nanhai Zhang
human	WB	Cell Oncol (Dordr); The POU2F1/miR-4490/USP22 axis regulates cell proliferation and metastasis in gastric cancer.; Authors - Yizhi Xiao
human	WB	iScience; MASTL is enriched in cancerous and pluripotent stem cells and influences OCT1/OCT4 levels.; Authors - Elisa Närvä ; KD Validated
mouse	WB	Mol Cell Endocrinol; MiR-451a attenuates free fatty acids-mediated hepatocyte steatosis by targeting the thyroid hormone responsive spot 14 gene.; Authors - Ni Zeng