Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, NCCIT cell|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human colon cancer tissue, human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10387-1-AP targets OCT1 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, pig samples.
|Tested Reactivity||human, pig|
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||OCT1 fusion protein Ag0586|
|Full Name||POU class 2 homeobox 1|
|Calculated molecular weight||76 kDa|
|Observed molecular weight||90 kDa|
|GenBank accession number||BC003571|
|Gene ID (NCBI)||5451|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The POU class 2 homeobox 1 (POU2F1), also known as octamer-binding transcription factor-1 (OCT-1), is a ubiquitous nuclear protein that regulates the transcription of target genes by binding to a cis-acting octamer element(5'-ATTTGCAT-3'). It participated in the regulation of housekeeping genes, including histone H2B and snRNAs, for cell differentiation and proliferation [PMID:3462701]. In addition, it functions as a cellular stress sensor that modulates activities of tissue-specific target genes involved in immune and inflammatory responses [PMID:16322220]. It also acts as a repressor for the cytokine-inducible, NF-kB-dependent expression of E-selectin and the vascular cell adhesion molecule during vascular inflammation. Otherwise, it can interact with nuclear hormone receptors, such as the retinoid X receptor, the thyroid hormone receptor and the glucocorticoid receptor, and influence their transcription activity[PMID:17192276].
|Product Specific Protocols|
|WB protocol for OCT1 antibody 10387-1-AP||Download protocol|
|IHC protocol for OCT1 antibody 10387-1-AP||Download protocol|
|IF protocol for OCT1 antibody 10387-1-AP||Download protocol|
|IP protocol for OCT1 antibody 10387-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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