TMEFF2 Polyclonal antibody

TMEFF2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11928-1-AP

Synonyms

HPP1, TENB2, TMEFF2, Tomoregulin 2, TPEF, TR, TR 2

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Validation Data Gallery

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  WB analysis using 11928-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11928-1-AP (TMEFF2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11928-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11928-1-AP (TMEFF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11928-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11928-1-AP (TMEFF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11928-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11928-1-AP (TMEFF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 11928-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11928-1-AP (TMEFF2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11928-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11928-1-AP (TMEFF2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HeLa cells, human brain tissue, PC-3 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

11928-1-AP targets TMEFF2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TMEFF2 fusion protein Ag2609
• Full Name: transmembrane protein with EGF-like and two follistatin-like domains 2
• Calculated Molecular Weight: 374 aa, 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC008973
• Gene Symbol: TMEFF2
• Gene ID (NCBI): 23671
• RRID: AB_2204477
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Immunol; Gene expression profiling and real-time PCR analyses identify novel potential cancer-testis antigens in multiple myeloma.; Authors - Condomines Maud M
human	IHC	Cancer Cell Int; TMEFF2 promoter hypermethylation is an unfavorable prognostic marker in gliomas.; Authors - Sidi Xie