- Phare
- Validé par KD/KO
Anticorps Monoclonal anti-P21
P21 Monoclonal Antibody for ELISA
Hôte / Isotype
Mouse / IgG2b
Réactivité testée
singe et plus (1)
Applications
WB, ELISA
Conjugaison
Non conjugué
CloneNo.
5F2F3
N° de cat : 60214-1-Ig
Synonymes
Galerie de données de validation
Applications testées
Dilution recommandée
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 1 publications below |
| WB | See 28 publications below |
Informations sur le produit
60214-1-Ig cible P21 dans les applications de WB, ELISA et montre une réactivité avec des échantillons singe
| Réactivité | singe |
| Réactivité citée | Humain |
| Hôte / Isotype | Mouse / IgG2b |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | P21 Protéine recombinante Ag0308 |
| Nom complet | cyclin-dependent kinase inhibitor 1A (p21, Cip1) |
| Masse moléculaire calculée | 21 kDa |
| Numéro d’acquisition GenBank | BC000312 |
| Symbole du gène | CDKN1A |
| Identification du gène (NCBI) | 1026 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
CDKN1A (p21, CIP1, WAF1), a cyclin-dependent kinase inhibitor, is necessary for proper control of the cell cycle and premature senescence. P21 is a cyclin-dependent kinase (CDK) inhibitor that suppresses proliferation by inhibiting CDK2 and CDK1 activity at the G1/S and G2/M transitions. P21 is a major mediator of p53 to induce cell cycle arrest in G1. p21 can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. P21 was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Overexpression of p21 decreases the expression levels of cell cycle progression genes and upregulates senescence-inducing genes.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for P21 antibody 60214-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother MiRNA-20a-5p promotes the growth of triple-negative breast cancer cells through targeting RUNX3. | ||
J Pathol NFIB promotes cell survival by directly suppressing p21 transcription in p53-mutated triple-negative breast cancer.
| ||
Virulence Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions. | ||
Aging (Albany NY) Transgenic mice overexpressing glia maturation factor-β, an oxidative stress inducible gene, show premature aging due to Zmpste24 down-regulation. | ||
J Biol Chem RNA binding protein RBM28 can translocate from the nucleolus to the nucleoplasm to inhibit the transcriptional activity of p53. | ||
Front Pharmacol Therapeutic Potential of CUDC-907 (Fimepinostat) for Hepatocarcinoma Treatment Revealed by Tumor Spheroids-Based Drug Screening |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ying (Verified Customer) (02-02-2021) | it is very good antibody.
|
FH Chunxia (Verified Customer) (02-02-2021) | Tissue culture
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