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SP1 Polyclonal antibody

SP1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat, monkey and More (2)





Cat no : 21962-1-AP


SP1, Sp1 transcription factor, Transcription factor Sp1, TSFP1

Tested Applications

Positive WB detected inJurkat cells, A431 cells, HEK-293 cells, HeLa cells, K-562 cells
Positive IP detected inA431 cells
Positive IHC detected inhuman lung cancer tissue, human colon tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21962-1-AP targets SP1 in WB, IP, IF, IHC, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse, rat, sheep, monkey, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SP1 fusion protein Ag16720
Full Name Sp1 transcription factor
Calculated Molecular Weight 785 aa, 81 kDa
Observed Molecular Weight 95-106 kDa
GenBank Accession NumberBC062539
Gene Symbol SP1
Gene ID (NCBI) 6667
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 is detected with MW 95-105 kDa and 65 kDa in this paper(PMID: 10618488).


Product Specific Protocols
WB protocol for SP1 antibody 21962-1-APDownload protocol
IHC protocol for SP1 antibody 21962-1-APDownload protocol
IF protocol for SP1 antibody 21962-1-APDownload protocol
IP protocol for SP1 antibody 21962-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

Authors - De Huang

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Disruption of MerTK increases the efficacy of checkpoint inhibitor by enhancing ferroptosis and immune response in hepatocellular carcinoma

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Sci Adv

Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss.

Authors - Xiaoqun Li

Nucleic Acids Res

High mobility group AT-hook 1 (HMGA1) is an important positive regulator of hepatitis B virus (HBV) that is reciprocally upregulated by HBV X protein.

Authors - Zhongliang Shen

Drug Des Devel Ther

Sichen Formula Ameliorates Lipopolysaccharide-Induced Acute Lung Injury via Blocking the TLR4 Signaling Pathways

Authors - Li-Shan Yan


AKT2 reduces IFNβ1 production to modulate antiviral responses and systemic lupus erythematosus.

Authors - Xin Zheng


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Anh (Verified Customer) (07-15-2021)

have some unspecific bands

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: adult mouse cardiomyocytes
SP1 Antibody Western Blot validation (1:500 dilution) in adult mouse cardiomyocytes (Cat no:21962-1-AP)

Hannah (Verified Customer) (06-08-2020)

Single band at predicted size with with 2 minute exposure

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: MRC5

David (Verified Customer) (01-15-2020)

Gave a single clear band at the expected molecular weight. Low protein input was used (10µg) for experimental reasons, hence the low antibody dilution required (1:100).

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: SH-SY5Y