SP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

115

Reviews (3)

Cat no : 21962-1-AP

Synonyms

SP1, Sp1 transcription factor, Transcription factor Sp1, TSFP1

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Validation Data Gallery

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  WB analysis using 21962-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21962-1-AP (SP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21962-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21962-1-AP (SP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21962-1-AP

  WB result of SP1 antibody (21962-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SP1 transfected HeLa cells.

• 

  WB analysis of HEK-293 using 21962-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21962-1-AP (SP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21962-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21962-1-AP (SP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 21962-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21962-1-AP (SP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 21962-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21962-1-AP (SP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 21962-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21962-1-AP (SP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21962-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21962-1-AP (SP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21962-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21962-1-AP (SP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 21962-1-AP

  IP result of anti-SP1 (IP:21962-1-AP, 6ug; Detection:21962-1-AP 1:500) with A431 cells lysate 2500ug.

• 

  IHC staining of human lung cancer using 21962-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21962-1-AP (SP1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 21962-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21962-1-AP (SP1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach cancer using 21962-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 21962-1-AP (SP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 21962-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 21962-1-AP (SP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 21962-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 21962-1-AP (SP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon using 21962-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 21962-1-AP (SP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 21962-1-AP

  Immunofluorescent analysis of HeLa cells using 21962-1-AP (SP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, A431 cells, HEK-293 cells, HeLa cells, K-562 cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human lung cancer tissue, human colon tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 22 publications below
• WB: See 106 publications below
• IHC: See 15 publications below
• IF: See 17 publications below
• IP: See 6 publications below
• CoIP: See 4 publications below
• ChIP: See 16 publications below

Product Information

21962-1-AP targets SP1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse, rat, pig, monkey, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SP1 fusion protein Ag16720
• Full Name: Sp1 transcription factor
• Calculated Molecular Weight: 785 aa, 81 kDa
• Observed Molecular Weight: 95-106 kDa
• GenBank Accession Number: BC062539
• Gene Symbol: SP1
• Gene ID (NCBI): 6667
• RRID: AB_10898171
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 is detected with MW 95-105 kDa and 65 kDa in this paper(PMID: 10618488).

Publications

Species	Application	Title
human	WB	Cell Res; Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.; Authors - De Huang
human	WB	Cell Rep Med; Disruption of MerTK increases the efficacy of checkpoint inhibitor by enhancing ferroptosis and immune response in hepatocellular carcinoma; Authors - Shun Wang
mouse	WB	Sci Adv; Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss.; Authors - Xiaoqun Li
human	WB	Nucleic Acids Res; High mobility group AT-hook 1 (HMGA1) is an important positive regulator of hepatitis B virus (HBV) that is reciprocally upregulated by HBV X protein.; Authors - Zhongliang Shen
mouse	WB,IP,IF	Redox Biol; Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke; Authors - Haiyun Guo
mouse	WB	Drug Des Devel Ther; Sichen Formula Ameliorates Lipopolysaccharide-Induced Acute Lung Injury via Blocking the TLR4 Signaling Pathways; Authors - Li-Shan Yan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anh (Verified Customer) (07-15-2021)

have some unspecific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: adult mouse cardiomyocytes

SP1 Antibody Western Blot validation (1:500 dilution) in adult mouse cardiomyocytes (Cat no:21962-1-AP)

FH

Hannah (Verified Customer) (06-08-2020)

Single band at predicted size with with 2 minute exposure

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: MRC5

FH

David (Verified Customer) (01-15-2020)

Gave a single clear band at the expected molecular weight. Low protein input was used (10µg) for experimental reasons, hence the low antibody dilution required (1:100).

• Applications: Western Blot,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: SH-SY5Y

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