Anticorps Polyclonal de lapin anti-ODF2

• Phare
• Validé par KD/KO

ODF2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

40

Avis (1)

N° de cat : 12058-1-AP

Synonymes

Cenexin, ODF2, ODF2/1, ODF2/2, ODF84, Outer dense fiber protein 2

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Galerie de données de validation

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  WB analysis of mouse testis using 12058-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12058-1-AP (ODF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12058-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12058-1-AP (ODF2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 12058-1-AP

  IP result of anti-ODF2 (IP:12058-1-AP, 5ug; Detection:12058-1-AP 1:500) with mouse testis tissue lysate 8000ug.

• 

  IHC staining of human breast cancer using 12058-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12058-1-AP (ODF2 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12058-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12058-1-AP (ODF2 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12058-1-AP

  Immunofluorescent analysis of HeLa cells using 12058-1-AP (ODF2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu pulmonaire de souris
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 19 publications below
• IHC: See 1 publications below
• IF: See 24 publications below

Informations sur le produit

12058-1-AP cible ODF2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ODF2 Protéine recombinante Ag2693
• Nom complet: outer dense fiber of sperm tails 2
• Masse moléculaire calculée: 763 aa, 89 kDa
• Poids moléculaire observé: 67-100 kDa
• Numéro d’acquisition GenBank: BC010629
• Symbole du gène: ODF2
• Identification du gène (NCBI): 4957
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ODF2 (outer dense fiber 2) is the major cenexin protein of the cytoskeleton of the sperm tail. In somatic cells, it is a component of the centrosome in which it is located in the appendages of the mother centriole. The gene for the cenexin protein, which was first identified as a protein marker of the mother centriole, was subsequently found to be identical to ODF2 and encodes transcripts cenexin 1 and cenexin 2. ODF2 is ubiquitously expressed in many cell types as various splicing variants. Additionally, ODF2 localizes to centrioles, basal bodies, and primary cilia, which are all structurally and functionally interconnected. ODF2 has 10 isoforms with MW 67-100 kDa.

Publications

Species	Application	Title
mouse	IF	Nature; Centrosome anchoring regulates progenitor properties and cortical formation.; Authors - Wei Shao
mouse	IF	Science; A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.; Authors - Chun So
mouse	IF	Cell; Structurally distinct ca(2+) signaling domains of sperm flagella orchestrate tyrosine phosphorylation and motility.; Authors - Jean-Ju Chung
human	IF	Nat Commun; Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.; Authors - Hai-Qing Tu
		Dev Cell; Rab11 endosomes contribute to mitotic spindle organization and orientation.; Authors - Heidi Hehnly
human	WB	J Exp Med; A DNAH17 missense variant causes flagella destabilization and asthenozoospermia.; Authors - Beibei Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jay (Verified Customer) (12-02-2019)

Methanol Fixation.Immunostaining result shows that Odf2 specifically marks the mother centriole in ciliated NIH3T3 cell (Odf2;red, GT335;green for primary cilia, DAPI;blue for nucleus).

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse NIH3T3

ODF2 Antibody Immunofluorescence, validation (1:200 dilution) in Mouse NIH3T3 (Cat no:12058-1-AP)

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