Dynamitin (1-83 AA) Polyclonal antibody

Dynamitin (1-83 AA) Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 10031-2-AP

Synonyms

DCTN 50, DCTN2, DCTN50, dynactin 2, dynactin 2 (p50), Dynactin subunit 2, DYNAMITIN, p50 dynamitin, RBP50

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Validation Data Gallery

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  WB analysis of mouse brain using 10031-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10031-2-AP (Dynamitin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 10031-2-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10031-2-AP (Dynamitin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 10031-2-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10031-2-AP (Dynamitin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 10031-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10031-2-AP (Dynamitin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10031-2-AP

  IP result of anti-Dynamitin (1-83 AA) (IP:10031-2-AP, 3ug; Detection:10031-2-AP 1:800) with mouse brain tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse testis tissue, mouse lung tissue, human testis tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

10031-2-AP targets Dynamitin (1-83 AA) in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: dynactin 2 (p50)
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC000718
• Gene Symbol: Dynamitin
• Gene ID (NCBI): 10540
• RRID: AB_2090613
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dynamitin is a 50 kDa protein containing a calmodulin binding domain, a putative ATPase domain and MacMARCKS-binding domain. This protein is a part of the dynactin complex believed to link the dynactin complex to membrane compartments. Its functions are tightly associated with dynein motor protein, thus extend to vesicle trafficking and membrane integrity. Dynamitin was named so because its overexpression causes dynactin complex which contains 10 subunits, to disassemble. Its N terminal 58 amino acid is for MacMARCKS binding and residues 59-83 is responsible for calmodulin binding. This antibody is against the N terminal 59aa of full length p50 dynamitin.

Publications

Species	Application	Title
mouse	WB	Nat Med; PMID: 19838201; Authors - Cheng Zhiyong Z

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

X (Verified Customer) (10-29-2020)

A very good p50 antibody to detect p50 at the righ MW by Western.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain/human brain lysates

Dynamitin (1-83 AA) Antibody Western Blot validation (1:2000 dilution) in mouse brain/human brain lysates (Cat no:10031-2-AP)