NEK2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NEK2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 24171-1-AP

Synonyms

NimA like protein kinase 1, Never in mitosis A-related kinase 2, NEK2A, HsPK21, HSPK 21

---

Validation Data Gallery

• 

  WB analysis using 24171-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24171-1-AP (NEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 24171-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24171-1-AP (NEK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24171-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24171-1-AP (NEK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 24171-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NEK2 antibody (24171-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Tested Applications

• Positive WB detected in: K-562 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 4 publications below

Product Information

24171-1-AP targets NEK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NEK2 fusion protein Ag21476
• Full Name: NIMA (never in mitosis gene a)-related kinase 2
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC043502
• Gene Symbol: NEK2
• Gene ID (NCBI): 4751
• RRID: AB_2687577
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Nat Commun; NEK2 inhibition triggers anti-pancreatic cancer immunity by targeting PD-L1.; Authors - Xiaozhen Zhang ; KD Validated
human	WB	Curr Biol; RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.; Authors - Long Jiang
mouse,human	IHC,WB	Biochim Biophys Acta Mol Cell Res; NEK2 overexpression aggravates IL-22-induced keratinocyte proliferation and cytokine level increases and IMQ-induced psoriasis-like dermatitis; Authors - Youhua Peng ; KD Validated
human,mouse	IHC	Int J Biol Sci; NEK2 plays an active role in Tumorigenesis and Tumor Microenvironment in Non-Small Cell Lung Cancer.; Authors - Rui Bai ; KD Validated
human	IHC,WB	Gene; NEK2 is associated with poor prognosis of clear cell renal cell carcinoma and promotes tumor cell growth and metastasis; Authors - Xiaoli Feng ; KD Validated
human	WB	Environ Toxicol; Circular forms of dedicator of cytokinesis 1 promotes breast cancer progression by derepressing never in mitosis related kinase 2 via sponging miR-128-3p; Authors - Zhaoxian Ni

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Sarah-Eve (Verified Customer) (06-02-2023)

Works very well WB dilution 1:1000 and for immunofluorescence of paraffin embedded tissue

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: Human pulmonary artery smooth muscle cells