Tested Applications
| Positive WB detected in | Jurkat cells, mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11517-1-AP targets CENPJ in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2078 Product name: Recombinant human CENPJ protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1033-1338 aa of BC024209 Sequence: RENTDLREEIKVMERFRLDAWKRAEAIESSLEVEKKDKLANTSVRFQNSQISSGTQVEKYKKNYLPMQGNPPRRSKSAPPRDLGNLDKGQAASPREPLEPLNFPDPEYKEEEEDQDIQGEISHPDGKVEKVYKNGCRVILFPNGTRKEVSADGKTITVTFFNGDVKQVMPDQRVIYYYAAAQTTHTTYPEGLEVLHFSSGQIEKHYPDGRKEITFPDQTVKNLFPDGQEESIFPDGTIVRVQRDGNKLIEFNNGQRELHTAQFKRREYPDGTVKTVYANGHQETKYRSGRIRVKDKEGNVLMDTEL Predict reactive species |
| Full Name | centromere protein J |
| Calculated Molecular Weight | 1338 aa, 153 kDa |
| Observed Molecular Weight | ~180 kDa |
| GenBank Accession Number | BC024209 |
| Gene Symbol | CENPJ |
| Gene ID (NCBI) | 55835 |
| RRID | AB_2244605 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HC77 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CENPJ, also named as CPAP, LAP and LIP1, belongs to the TCP10 family. It plays an important role in cell division and centrosome function by participating in centriole duplication. CENPJ inhibits microtubule nucleation from the centrosome.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CENPJ antibody 11517-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CENPJ (CPAP) polyclonal antibody (Cat# 11517-1-AP) has 63 citations across high-impact journals including Nature, Science, Cell, Nature Communications, Neuron, Current Biology, EMBO Journal, J Cell Biol, and eLife. Research fields span centriole biogenesis, centrosome integrity, ciliogenesis, cell division, microtubule organization, autophagy, neurodevelopment (microcephaly), and cancer biology.
| Species | Application | Title |
|---|---|---|
Science Cell biology. Reversible centriole depletion with an inhibitor of Polo-like kinase 4. | ||
Cell Time-series reconstruction of the molecular architecture of human centriole assembly | ||
Cancer Discov Epigenetic Regulation of Chromosomal Instability by EZH2 Methyltransferase. | ||
Nat Cell Biol The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis. |
Reviews
What customers say
Based on one available review, a user (Jeppesen, 2026) rated this antibody 2 out of 5 stars. They tested it at a 1:100 dilution for immunohistochemistry and whole-tissue immunofluorescence on mouse gonad sections (lot 00089139) and reported that the antibody did not work in either application. Overall, this single review reflects a negative experience with the product's performance in mouse tissue.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Chinzorig (Verified Customer) (02-01-2026) | The antibody did not work in mouse tissue sections and whole-tissue IF.
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