Anticorps Polyclonal de lapin anti-NUB1

• Phare
• Validé par KD/KO

NUB1 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 14343-1-AP

Synonymes

BS4, NEDD8 ultimate buster 1, NUB1, NUB1L, NYREN18

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Galerie de données de validation

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  WB analysis of HEK-293 using 14343-1-AP

  WB result of NUB1 antibody (14343-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUB1 transfected HEK-293 cells.

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  WB analysis using 14343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14343-1-AP (NUB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14343-1-AP

  IP result of anti-NUB1 (IP:14343-1-AP, 4ug; Detection:14343-1-AP 1:600) with HEK-293 cells lysate 1575 ug.

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  IF Staining of HeLa using 14343-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUB1 antibody (14343-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Raji
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

14343-1-AP cible NUB1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NUB1 Protéine recombinante Ag5668
• Nom complet: negative regulator of ubiquitin-like proteins 1
• Masse moléculaire calculée: 71 kDa
• Poids moléculaire observé: 69 kDa
• Numéro d’acquisition GenBank: BC046354
• Symbole du gène: NUB1
• Identification du gène (NCBI): 51667
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Proteasome-associated protein NEDD8 ultimate buster 1 (NUB1) is an interferon-inducible protein that down-regulates NEDD8 expression and its conjugation system. Over-expression of NUB1 induces a growth-inhibitory effect in cells, and it mediates anti-proliferative actions and apoptosis in renal cell carcinoma cells through cell-cycle regulation. Expression of NUB1 leads to decreased modification of p53 with NEDD8 and stimulation of p53 ubiquitination. Recent finding revealed that NUB1, by regulating GSK3β levels, modulates tau phosphorylation and aggregation, and is a key player in neurodegeneration associated with tau pathology.

Publications

Species	Application	Title
mouse	WB,IF	Biochem Biophys Res Commun; The role of NUB1 in α-synuclein degradation in Lewy body disease model mice.; Authors - Kunikazu Tanji
human	IHC	Neuropathology; Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases.; Authors - Fumiaki Mori
human	WB	Brain Pathol; Phosphorylated NUB1 distinguishes α-synuclein in Lewy bodies from that in glial cytoplasmic inclusions in multiple system atrophy; Authors - Kunikazu Tanji

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