NEDD8 Polyclonal antibody

• Featured Product
• KD/KO Validated

NEDD8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 16777-1-AP

Synonyms

cul nedd8, Nedd 8, NEDD8, Neddylin, Ubiquitin like protein Nedd8

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Validation Data Gallery

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  WB analysis of mouse brain using 16777-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16777-1-AP (NEDD8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 16777-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16777-1-AP (NEDD8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16777-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16777-1-AP (NEDD8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16777-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16777-1-AP (NEDD8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 16777-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16777-1-AP (NEDD8 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 16777-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16777-1-AP (NEDD8 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human heart tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

16777-1-AP targets NEDD8 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

• Tested Reactivity: human, mouse, rat, monkey
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NEDD8 fusion protein Ag10154
• Full Name: neural precursor cell expressed, developmentally down-regulated 8
• Calculated Molecular Weight: 81 aa, 9 kDa
• Observed Molecular Weight: 6-10 kDa
• GenBank Accession Number: BC104664
• Gene Symbol: NEDD8
• Gene ID (NCBI): 4738
• RRID: AB_10598467
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEDD8 (neural precursor cell-expressed developmentally down-regulated gene 8) is a small ubiquitin-like protein that shares 60% sequence identity and 80% homology with ubiquitin [PMID:9353319]. NEDD8 is conjugated to substrate proteins in a process known as neddylation. NEDD8 forms a thioester bond with APPBP1-Uba3, the E1 enzyme. Activated NEDD8 then transfers to Ubc12, the E2 enzyme . Ultimately, E2 loaded with NEDD8 binds a substrate protein lysine residue and forms an isopeptide bond by E3 ligase [PMID:18802447].

Publications

Species	Application	Title
		Nat Neurosci; Identification of NUB1 as a suppressor of mutant Huntington toxicity via enhanced protein clearance.; Authors - Lu Boxun B
human	WB	J Transl Med; TC2N inhibits distant metastasis and stemness of breast cancer via blocking fatty acid synthesis; Authors - Xiang-Lin Hao
rat	IF,WB	Neuropsychopharmacology; Synaptic control of DNA methylation involves activity-dependent degradation of DNMT3A1 in the nucleus.; Authors - Gonca Bayraktar ; KD Validated
		J Med Chem; Halo and Pseudohalo Gold(I)-NHC Complexes Derived from 4,5-Diarylimidazoles with Excellent in Vitro and in Vivo Anticancer Activity Against HCC.; Authors - Mianli Bian
human	WB	Front Bioeng Biotechnol; Let-7i-5p Regulation of Cell Morphology and Migration Through Distinct Signaling Pathways in Normal and Pathogenic Urethral Fibroblasts.; Authors - Kaile Zhang
human	IHC	J Exp Clin Cancer Res; Neddylation activated TRIM25 desensitizes triple-negative breast cancer to paclitaxel via TFEB-mediated autophagy; Authors - Bowen Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jose (Verified Customer) (09-14-2021)

Strong signal

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000