SNCAIP Polyclonal antibody

SNCAIP Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17818-1-AP

Synonyms

SNCAIP, Sph1, Synphilin 1, SYPH1

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Validation Data Gallery

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  WB analysis of mouse brain using 17818-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17818-1-AP (SNCAIP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 17818-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 17818-1-AP (SNCAIP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17818-1-AP

  IP result of anti-SNCAIP (IP:17818-1-AP, 4ug; Detection:17818-1-AP 1:300) with mouse brain tissue lysate 3600ug.

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  IHC staining of human kidney using 17818-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17818-1-AP (SNCAIP Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 17818-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17818-1-AP (SNCAIP Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, fetal human brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 17818-1-AP is SNCAIP Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNCAIP fusion protein Ag12147
• Full Name: synuclein, alpha interacting protein
• Calculated Molecular Weight: 603aa,67 kDa; 1016aa,111 kDa
• Observed Molecular Weight: 100 kDa, 75 kDa
• GenBank Accession Number: BC094759
• Gene Symbol: SNCAIP
• Gene ID (NCBI): 9627
• RRID: AB_2878444
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNCAIP, also named as Synphilin-1 or Alpha-synuclein-interacting protein, is a 919 amino acid protein, which contains six ANK repeats. SNCAIP is detected in brain and is widely expressed, with highest levels in brain, heart and placenta. SNCAIP localizes in the cytoplasm and inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. SNCAIP has an association with Parkinson disease.

Publications

Species	Application	Title
human	WB	Neurosci Lett; Synphilin-1 has neuroprotective effects on MPP+-induced Parkinson's disease model cells by inhibiting ROS production and apoptosis.; Authors - Takeo Shishido