UBE2K Polyclonal antibody

UBE2K Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11834-3-AP

Synonyms

E2-25K, HIP 2, HIP2, HYPG, LIG, UBE2K, Ubiquitin carrier protein, Ubiquitin protein ligase

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Validation Data Gallery

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  WB analysis using 11834-3-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11834-3-AP (UBE2K antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11834-3-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11834-3-AP (UBE2K antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11834-3-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11834-3-AP (UBE2K antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 11834-3-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11834-3-AP (UBE2K antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human thyroid cancer using 11834-3-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11834-3-AP (UBE2K antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 11834-3-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11834-3-AP (UBE2K antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse testis tissue, human brain tissue, rat brain tissue, rat testis tissue
• Positive IHC detected in: human gliomas tissue, human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

11834-3-AP targets UBE2K in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBE2K fusion protein Ag2415
• Full Name: ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)
• Calculated Molecular Weight: 200 aa, 22 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC022804
• Gene Symbol: UBE2K
• Gene ID (NCBI): 3093
• RRID: AB_2241168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Int J Oncol; UBE2K regulated by IGF2BP3 promotes cell proliferation and stemness in pancreatic ductal adenocarcinoma; Authors - Wen Fu
human	WB	Biochem Biophys Res Commun; Ixazomib inhibits myeloma cell proliferation by targeting UBE2K.; Authors - Qingqing Wang
human	WB	Biomed Environ Sci; Ezrin Promotes the Proliferation, Migration, and Invasion of Ovarian Cancer Cells.; Authors - Mo Juan Li
rat	WB	Neuropathology; Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation.; Authors - Kazuhiko Watabe