Anticorps Polyclonal de lapin anti-NFKB1,p105,p50

NFKB1,p105,p50 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (6)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA

Conjugaison

Non conjugué

Publications

172

Avis (2)

N° de cat : 14220-1-AP

Synonymes

NFKB1, NF kappa B, KBF1, EBP-1, EBP 1

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Galerie de données de validation

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  WB analysis using 14220-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14220-1-AP (NFKB1,p105,p50 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14220-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14220-1-AP (NFKB1,p105,p50 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 14220-1-AP

  IP result of anti-NFKB1,p105,p50 (IP:14220-1-AP, 4ug; Detection:14220-1-AP 1:3000) with Jurkat cells lysate 2280 ug.

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  IF Staining of HepG2 using 14220-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14220-1-AP (NFKB1,p105,p50 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 14220-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NFKB1 antibody (14220-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 14220-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human NFKB1,p105,p50 (14220-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules Jurkat, cellules MG U-87, cellules Raji
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 145 publications below
• IHC: See 13 publications below
• IF: See 24 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below
• ChIP: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

14220-1-AP cible NFKB1,p105,p50 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, bovin, canin, Humain, porc, poulet, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NFKB1,p105,p50 Protéine recombinante Ag5458
• Nom complet: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 50 kDa, 105 kDa
• Numéro d’acquisition GenBank: BC051765
• Symbole du gène: NFKB1
• Identification du gène (NCBI): 4790
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NFkB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFkB is activated by various intra- and extracellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo- and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. This antibody can bind both p105 and p50 isoforms of NFKB1.

Publications

Species	Application	Title
human		Nat Immunol; NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.; Authors - Di Huang
human	IF	Nucleic Acids Res; p38-mediated FOXN3 phosphorylation modulates lung inflammation and injury through the NF-κB signaling pathway; Authors - Xinxing Zhu
mouse	WB	Nat Microbiol; Chloroquine ameliorates carbon tetrachloride-induced acute liver injury in mice via the concomitant inhibition of inflammation and induction of apoptosis; Authors - Tomas Doyle
mouse		Sci Adv; Microglial Ffar4 deficiency promotes cognitive impairment in the context of metabolic syndrome; Authors - Wei Wang
mouse	WB	Biomaterials; A multifunctional anti-inflammatory drug that can specifically target activated macrophages, massively deplete intracellular H2O2, and produce large amounts CO for a highly efficient treatment of osteoarthritis.; Authors - Guangzhen Yang
human	WB	EMBO J; The p53-inducible long noncoding RNA TRINGS protects cancer cells from necrosis under glucose starvation.; Authors - Muhammad Riaz Khan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Vikas (Verified Customer) (04-23-2024)

Used NF-KB antibody for western 1:1000 dilution, worked very well, highly recommended product.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: SH-SY5Y cell line

FH

Ane (Verified Customer) (09-18-2023)

This antibody was used to detect p50 at different cell compartments and worked fine to dectect both phosphorilated and unphosphorilated forms of p50 in all cell compartments

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HCT-15 cell line

NFKB1,p105,p50 Antibody Western Blot validation (1:1000 dilution) in HCT-15 cell line (Cat no:14220-1-AP)

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