CXCL8/IL-8 Polyclonal antibody

CXCL8/IL-8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

21

Cat no : 17038-1-AP

Synonyms

CXCL8, IL8, IL-8, CXCL 8, C-X-C motif chemokine 8

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Validation Data Gallery

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  WB analysis using 17038-1-AP

  PMA, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 17038-1-AP (CXCL8/IL-8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 17038-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17038-1-AP (CXCL8/IL-8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 17038-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17038-1-AP (CXCL8/IL-8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 17038-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17038-1-AP (CXCL8/IL8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 17038-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17038-1-AP (CXCL8/IL8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: PMA, LPS and Brefeldin A treated THP-1 cells
• Positive IHC detected in: human tonsillitis tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 7 publications below
• IF: See 4 publications below
• ELISA: See 1 publications below

Product Information

17038-1-AP targets CXCL8/IL-8 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CXCL8/IL-8 fusion protein Ag10552
• Full Name: interleukin 8
• Calculated Molecular Weight: 99 aa, 11 kDa
• Observed Molecular Weight: 11 kDa
• GenBank Accession Number: BC013615
• Gene Symbol: IL-8
• Gene ID (NCBI): 3576
• RRID: AB_2126217
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin 8 (IL-8), also known as CXCL8, which is a member of the CXC chemokine family. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL-8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL-8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL-8 is also known to be a potent promoter of angiogenesis. IL-8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.

Publications

Species	Application	Title
human		Mol Cancer; RIPK3 activation induces TRIM28 derepression in cancer cells and enhances the anti-tumor microenvironment.; Authors - Han-Hee Park
human		EMBO Rep; LncRNA GUARDIN suppresses cellular senescence through a LRP130-PGC1α-FOXO4-p21-dependent signaling axis.; Authors - Xuedan Sun
human	IHC,IF	Cell Death Discov; Endometrial stromal cell ferroptosis promotes angiogenesis in endometriosis.; Authors - Guojing Li
human		Cell Death Dis; Necroptosis promotes cell-autonomous activation of proinflammatory cytokine gene expression.; Authors - Kezhou Zhu
	IHC	Int Immunopharmacol; Bomidin attenuates inflammation of periodontal ligament stem cells and periodontitis in mice via inhibiting ferroptosis; Authors - Wei Wu
human	IHC	J Neurooncol; Radiotherapy induces an immediate inflammatory reaction in malignant glioma: a clinical microdialysis study.; Authors - Pedram Tabatabaei