IkB Alpha Polyclonal antibody

IkB Alpha Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig, rat and More (3)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10268-1-AP

Synonyms

I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, Ikβ-α, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, HL-60 cells, A431 cells, pig liver tissue, HEK-293 cells, HepG2 cells, NIH/3T3 cells, PC-12 cells, NIH/T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, mouse kidney tissue, human stomach tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10268-1-AP targets IkB Alpha in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Cited Reactivityhuman, mouse, rat, bovine, chicken, ducks, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IkB Alpha fusion protein Ag0345
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC002601
Gene symbol NFKBIA
Gene ID (NCBI) 4792
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

Protocols

Product Specific Protocols
WB protocol for IkB Alpha antibody 10268-1-APDownload protocol
IHC protocol for IkB Alpha antibody 10268-1-APDownload protocol
IF protocol for IkB Alpha antibody 10268-1-APDownload protocol
IP protocol for IkB Alpha antibody 10268-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Cell Biol

Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.

Authors - Li Wang
mouseWB

Sci Adv

Elevated type I interferon responses potentiate metabolic dysfunction, inflammation, and accelerated aging in mtDNA mutator mice.

Authors - Yuanjiu Lei
mouseWB

Nat Commun

USP12 downregulation orchestrates a protumourigenic microenvironment and enhances lung tumour resistance to PD-1 blockade.

Authors - Zhaojuan Yang
humanWB

Autophagy

Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.

Authors - Qichao Huang
pigWB

Nucleic Acids Res

The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.

Authors - Xin Zong
humanWB

EMBO J

The p53-inducible long noncoding RNA TRINGS protects cancer cells from necrosis under glucose starvation.

Authors - Muhammad Riaz Khan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

CHAO (Verified Customer) (01-30-2020)

Weak bands for the heart lysates at basal state.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart