NFKB2,p100 Polyclonal antibody

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  WB analysis using 10409-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10409-2-AP (NFKB2,p100 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 using 10409-2-AP

  WB result of NFKB2,p100 antibody (10409-2-AP; 1:60000; room temperature for 1.5 hours) with wild-type and sg-NFKB2,p100 transfected HAP1 cells.

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  IP experiment of K-562 using 10409-2-AP

  IP result of anti-NFKB2,p100 (IP:10409-2-AP, 4ug; Detection:10409-2-AP 1:600) with K-562 cells lysate 1200ug.

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  IHC staining of human liver cancer using 10409-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10409-2-AP (NFKB2,p100 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 10409-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10409-2-AP (NFKB2,p100 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 10409-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10409-2-AP (NFKB2,p100 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 10409-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10409-2-AP (NFKB2,p100 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of U2OS using 10409-2-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using NFKB2 antibody (10409-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

NFKB2,p100 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10409-2-AP

Publications(21)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

NFKB2, DNA binding factor KBF2, DNA-binding factor KBF2, H2TF1, LYT 10

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Tested Applications

• Positive WB detected in: HeLa cells, HAP1 cells, K-562 cells, NIH/3T3 cells, RAW 264.7 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human pancreas cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 16 publications below
• IHC: See 8 publications below
• IF: See 2 publications below

Product Information

10409-2-AP targets NFKB2,p100 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NFKB2,p100 fusion protein Ag0662
• Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC002844
• Gene Symbol: NFKB2
• Gene ID (NCBI): 4791
• ENSEMBL Gene ID: ENSG00000077150
• RRID: AB_2151261
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q00653
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody can bind p100 isoform of NFKB2 spefically, but not p52 isoform.

Publications

Species	Application	Title
mouse	WB	Nat Commun; A proteomics landscape of circadian clock in mouse liver.; Authors - Yunzhi Wang
human	WB	Nat Commun; A translational program that suppresses metabolism to shield the genome.; Authors - Nathan C Balukoff
mouse	WB	J Hazard Mater; TiO2 nanosheets promote the transformation of vascular smooth muscle cells into foam cells in vitro and in vivo through the up-regulation of nuclear factor kappa B subunit 2.; Authors - Shuang Li
human	WB	PLoS Pathog; SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.; Authors - Muyu Xu
human	WB,IHC	Biochim Biophys Acta Mol Basis Dis; LRRC8A as a central mediator promotes colon cancer metastasis by regulating PIP5K1B/PIP2 pathway; Authors - Haifeng Zhang
human	WB	Cell Death Dis; Identification of 11(13)-dehydroivaxillin as a potent therapeutic agent against non-Hodgkin's lymphoma.; Authors - Xinhua Xiao