NFKB2 Polyclonal antibody

• Featured Product
• KD/KO Validated

NFKB2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 10409-2-AP

Synonyms

DNA binding factor KBF2, H2TF1, LYT 10, LYT10, NF kappa B, NFKB2, NFKB2,p100, Oncogene Lyt 10, p100

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Validation Data Gallery

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  WB analysis of multi-cells using 10409-2-AP

  WB result of 10409-2-AP (NFKB2 antibody).

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  WB analysis of HeLa using 10409-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10409-2-AP (NFKB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10409-2-AP

  IP Result of anti-NFKB2 (IP:10409-2-AP, 4ug; Detection:10409-2-AP 1:600) with K-562 cells lysate 1200ug.

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  IHC staining of human liver cancer using 10409-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10409-2-AP (NFKB2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas cancer using 10409-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10409-2-AP (NFKB2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 10409-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10409-2-AP (NFKB2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas cancer using 10409-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10409-2-AP (NFKB2 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 10409-2-AP

  Immunofluorescent analysis of HeLa cells using 10409-2-AP (NFKB2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: multi-cells, HeLa cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human pancreas cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Product Information

10409-2-AP targets NFKB2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NFKB2 fusion protein Ag0662
• Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
• Calculated molecular weight: 97 kDa
• Observed molecular weight: 110 kDa
• GenBank accession number: BC002844
• Gene symbol: NFKB2
• Gene ID (NCBI): 4791
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody recognizes both p52 and p100.

Publications

Species	Application	Title
mouse	WB	Nat Commun; A proteomics landscape of circadian clock in mouse liver.; Authors - Yunzhi Wang
human	WB	Nat Commun; A translational program that suppresses metabolism to shield the genome.; Authors - Nathan C Balukoff
mouse	WB	J Hazard Mater; TiO2 nanosheets promote the transformation of vascular smooth muscle cells into foam cells in vitro and in vivo through the up-regulation of nuclear factor kappa B subunit 2.; Authors - Shuang Li
human	WB	PLoS Pathog; SHMT2 and the BRCC36/BRISC deubiquitinase regulate HIV-1 Tat K63-ubiquitylation and destruction by autophagy.; Authors - Muyu Xu
human	WB	Cell Death Dis; Identification of 11(13)-dehydroivaxillin as a potent therapeutic agent against non-Hodgkin's lymphoma.; Authors - Xinhua Xiao
human	WB	Mol Ther Nucleic Acids; circPUM1 Promotes Tumorigenesis and Progression of Ovarian Cancer by Sponging miR-615-5p and miR-6753-5p.; Authors - Xue Guan