Anticorps Monoclonal anti-NF-H/NF200

NF-H/NF200 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

12

CloneNo.

1A3C7

N° de cat : 60331-1-Ig

Synonymes

200 kDa neurofilament protein, NEFH, NEH, neurofilament, NF, NF H, NF200, NF-H

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Galerie de données de validation

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  WB analysis of pig cerebellum using 60331-1-Ig

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 60331-1-Ig (NF-H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 60331-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60331-1-Ig (NF-H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 60331-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60331-1-Ig (NF-H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat cerebellum using 60331-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 60331-1-Ig (NF-H antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 60331-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NF-H/NF200 antibody (60331-1-Ig, Clone: 1A3C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of SH-SY5Y using 60331-1-Ig

  Immunofluorescent analysis of SH-SY5Y cells using 60331-1-Ig (NF200 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

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  FC experiment of SH-SY5Y using 60331-1-Ig

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Anti-Human NF-H/NF200 (60331-1-Ig, Clone:1A3C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu de cervelet de porc, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cervelet de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SH-SY5Y, tissu cérébral de rat
• Résultats positifs en cytométrie: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:4000-1:16000
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 3 publications below
• IF: See 10 publications below

Informations sur le produit

60331-1-Ig cible NF-H/NF200 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NF-H/NF200 Protéine recombinante Ag13517
• Nom complet: neurofilament, heavy polypeptide
• Masse moléculaire calculée: 112 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC014185
• Symbole du gène: NEFH
• Identification du gène (NCBI): 4744
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NEFH, also named as KIAA0845 and NFH, Belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody recognize NEFH only.

Publications

Species	Application	Title
rat	IF	Bioact Mater; Li-Mg-Si bioceramics provide a dynamic immuno-modulatory and repair-supportive microenvironment for peripheral nerve regeneration; Authors - Yiting Sun
mouse	IF	ACS Nano; Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia; Authors - Shishi Shen
human,mouse	IF	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
rat	IF	Biomaterials; Oriented nanofibrous P(MMD-co-LA)/Deferoxamine nerve scaffold facilitates peripheral nerve regeneration by regulating macrophage phenotype and revascularization.; Authors - Xianzhen Dong
rat	IF	J Neuroinflammation; Apelin alleviated neuroinflammation and promoted endogenous neural stem cell proliferation and differentiation after spinal cord injury in rats.; Authors - Qing Liu
mouse	IHC	Front Cell Neurosci; Ursolic Acid Ameliorates Spinal Cord Injury in Mice by Regulating Gut Microbiota and Metabolic Changes.; Authors - Zi-Jie Rong

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