NF-L Polyclonal antibody

NF-L Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA, FC (Intra)

Conjugate

Unconjugated

Cat no : 12998-1-AP

Synonyms

68 kDa neurofilament protein, CMT1F, CMT2E, NEFL, NF, NF L, NF68, NFL, NF-L



Tested Applications

Positive WB detected inSH-SY5Y cells, Neuro-2a cells, mouse brain tissue, C6 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells, mouse brain tissue
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12998-1-AP targets NF-L in WB, IP, IF, IHC, ELISA, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NF-L fusion protein Ag3667
Full Name neurofilament, light polypeptide
Calculated Molecular Weight 543 aa, 62 kDa
Observed Molecular Weight 65-68 kDa, 140-160 kDa
GenBank Accession NumberBC039237
Gene Symbol NF-L
Gene ID (NCBI) 4747
RRIDAB_10597388
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NEFL, also named as NF68 and NF-L, belongs to the intermediate filament family. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H, which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M, 145-160 kDa and NF-H, 200-220 kDa.This antibody is specific to NEFL. It has a very weak reaction to NEFM.

Protocols

Product Specific Protocols
WB protocol for NF-L antibody 12998-1-APDownload protocol
IHC protocol for NF-L antibody 12998-1-APDownload protocol
IF protocol for NF-L antibody 12998-1-APDownload protocol
IP protocol for NF-L antibody 12998-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

Mixed Lineage Kinase Domain-like Protein MLKL Breaks Down Myelin following Nerve Injury.

Authors - Zhengxin Ying
mouseWB

Mater Today Bio

Injectable hyaluronic acid hydrogel loaded with BMSC and NGF for traumatic brain injury treatment.

Authors - Luyu Wang
mouseWB

Mater Today Bio

In situ forming and biocompatible hyaluronic acid hydrogel with reactive oxygen species-scavenging activity to improve traumatic brain injury repair by suppressing oxidative stress and neuroinflammation.

Authors - Dan Zhang
ratWB,IF

Adv Sci (Weinh)

An Important Function of Petrosiol E in Inducing the Differentiation of Neuronal Progenitors and in Protecting Them against Oxidative Stress.

Authors - Jing Liu

Front Mol Neurosci

Achaete-Scute Homolog 1 Expression Controls Cellular Differentiation of Neuroblastoma.

Authors - Mumtaz Kasim
mouseWB

Hum Mol Genet

Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.

Authors - Peng-Peng Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Max (Verified Customer) (02-24-2022)

strong and specific labeling of NfL in hamster brain lysate on western blot

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Hamster brain lysate