mouse brain and rat brain tissues were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse brain and rat brain tissues were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain tissue using 20664-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 20664-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 20664-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
human brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 20664-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
rat brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of rat brain using 20664-1-AP
IP result of anti-NF-M-Specific (IP:20664-1-AP, 4ug; Detection:20664-1-AP 1:1000) with rat brain tissue lysate 4000ug.
IP result of anti-NF-M-Specific (IP:20664-1-AP, 4ug; Detection:20664-1-AP 1:1000) with rat brain tissue lysate 4000ug.
IHC staining of human brain using 20664-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:200 (under 10x lens).
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:200 (under 10x lens).
IHC staining of human brain using 20664-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:200 (under 40x lens).
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:200 (under 40x lens).
IHC staining of rat brain using 20664-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:50.
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:50.
IF Staining of SH-SY5Y using 20664-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 20664-1-AP (NF-M-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 20664-1-AP (NF-M-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
FC experiment of PC-12 using 20664-1-AP
1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-M-Specific (20664-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-M-Specific (20664-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse brain tissue, rat brain tissue, human brain tissue
Positive IP detected in
rat brain tissue
Positive IHC detected in
human brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
SH-SY5Y cells
Positive FC (Intra) detected in
PC-12 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:3000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. The antibody is specific to NEFM.
mouse brain and rat brain tissues were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain tissue using 20664-1-AP
WB result of 20664-1-AP.
WB analysis of mouse brain using 20664-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 20664-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 20664-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 20664-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 20664-1-AP (NF-M-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human brain using 20664-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:200 (under 10x lens).
IHC staining of human brain using 20664-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:200 (under 40x lens).
IHC staining of rat brain using 20664-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:50.
IHC staining of rat brain using 20664-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20664-1-AP (NF-M-Specific antibody at dilution of 1:50.
IP Figures
IP experiment of rat brain using 20664-1-AP
IP result of anti-NF-M-Specific (IP:20664-1-AP, 4ug; Detection:20664-1-AP 1:1000) with rat brain tissue lysate 4000ug.
IF/ICC Figures
IF Staining of SH-SY5Y using 20664-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 20664-1-AP (NF-M-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
FC (INTRA) Figures
FC experiment of PC-12 using 20664-1-AP
1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human NF-M-Specific (20664-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
NF-M-Specific Polyclonal antibody
Catalog Number
20664-1-AP
Citations
2
Dilutions
WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
