• Featured Product
  • KD/KO Validated

PIN1 Polyclonal antibody

PIN1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10495-1-AP

Synonyms

DOD, PIN1, PPIase Pin1, Rotamase Pin1, UBL5



Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, PC-12 cells, mouse brain tissue, rat brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas cancer tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10495-1-AP targets PIN1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PIN1 fusion protein Ag0767
Full Name peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC002899
Gene symbol PIN1
Gene ID (NCBI) 5300
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

• PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus. Pin1 inhibition simultaneously blocks multiple cancer pathways, disrupts the desmoplastic and immunosuppressive TME, and upregulates PD-L1 and ENT1, rendering pancreatic ductal adenocarcinoma (PDAC) eradicable by immunochemotherapy (PMID: 34388391).

Protocols

Product Specific Protocols
WB protocol for PIN1 antibody 10495-1-APDownload protocol
IHC protocol for PIN1 antibody 10495-1-APDownload protocol
IF protocol for PIN1 antibody 10495-1-APDownload protocol
IP protocol for PIN1 antibody 10495-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

J Pineal Res

Melatonin directly binds and inhibits death-associated protein kinase 1 function in Alzheimer's disease.

Authors - Dongmei Chen
humanWB,IF

J Hazard Mater

Cobalt induces neurodegenerative damages through Pin1 inactivation in mice and human neuroglioma cells.

Authors - Fuli Zheng
humanWB

Theranostics

SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinoma.

Authors - Ling Lin
humanWB

Nucleic Acids Res

Pin1 promotes GR transactivation by enhancing recruitment to target genes.

Authors - Poolman Toryn M TM
  • KD Validated

J Med Chem

Halo and Pseudohalo Gold(I)-NHC Complexes Derived from 4,5-Diarylimidazoles with Excellent in Vitro and in Vivo Anticancer Activity Against HCC.

Authors - Mianli Bian
mouseWB

Metabolism

Prolyl isomerase Pin1 interacts with adipose triglyceride lipase and negatively controls both its expression and lipolysis.

Authors - Yusuke Nakatsu
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

  • Applications: Other