Validation Data Gallery
|Positive WB detected in||A549 cells, HEK-293 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, PC-12 cells, mouse brain tissue, rat brain tissue|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human pancreas cancer tissue, human renal cell carcinoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HEK-293 cells, NIH/3T3 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10495-1-AP targets PIN1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PIN1 fusion protein Ag0767|
|Full Name||peptidylprolyl cis/trans isomerase, NIMA-interacting 1|
|Calculated molecular weight||18 kDa|
|Observed molecular weight||18 kDa|
|GenBank accession number||BC002899|
|Gene ID (NCBI)||5300|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
• PIN1(Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1) is essential for mitosis progression in yeast cells and is hypothesized to perform the same role in mammalian cells. It might regulate cellular processes distinct from the cell cycle itself, such as terminal differentiation through a modulation of differentiation-specific gene expression(PMID:20801874). It colocalizes with NEK6 in the nucleus. Pin1 inhibition simultaneously blocks multiple cancer pathways, disrupts the desmoplastic and immunosuppressive TME, and upregulates PD-L1 and ENT1, rendering pancreatic ductal adenocarcinoma (PDAC) eradicable by immunochemotherapy (PMID: 34388391).
|Product Specific Protocols|
|WB protocol for PIN1 antibody 10495-1-AP||Download protocol|
|IHC protocol for PIN1 antibody 10495-1-AP||Download protocol|
|IF protocol for PIN1 antibody 10495-1-AP||Download protocol|
|IP protocol for PIN1 antibody 10495-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Pineal Res
Melatonin directly binds and inhibits death-associated protein kinase 1 function in Alzheimer's disease.
J Hazard Mater
Cobalt induces neurodegenerative damages through Pin1 inactivation in mice and human neuroglioma cells.
SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinoma.
Nucleic Acids Res
Pin1 promotes GR transactivation by enhancing recruitment to target genes.
J Med Chem
Halo and Pseudohalo Gold(I)-NHC Complexes Derived from 4,5-Diarylimidazoles with Excellent in Vitro and in Vivo Anticancer Activity Against HCC.
Prolyl isomerase Pin1 interacts with adipose triglyceride lipase and negatively controls both its expression and lipolysis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Carly (Verified Customer) (11-17-2020)
Tested using EDTA plasma on an antibody microarray