Anticorps Polyclonal de lapin anti-NF-H/NF200
NF-H/NF200 Polyclonal Antibody for WB, IF, IHC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (1)
Applications
WB, IHC, IF-P, ELISA
Conjugaison
Non conjugué
N° de cat : 18934-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | tissu cérébral de souris, tissu cérébral de rat |
Résultats positifs en IHC | tissu cérébral de rat, tissu de gliome humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF-P | tissu cérébral de rat, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochimie (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 17 publications below |
IHC | See 12 publications below |
IF | See 23 publications below |
Informations sur le produit
18934-1-AP cible NF-H/NF200 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | rat, canin, Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | NF-H/NF200 Protéine recombinante Ag13517 |
Nom complet | neurofilament, heavy polypeptide |
Masse moléculaire calculée | 112 kDa |
Poids moléculaire observé | 200 kDa, 140-160 kDa |
Numéro d’acquisition GenBank | BC014185 |
Symbole du gène | NF-H |
Identification du gène (NCBI) | 4744 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
NEFH (NF200), also named as KIAA0845 and NFH, belongs to the intermediate filament family. It has an important function in mature axons that is not subserved by the two smaller NF proteins. Neurofilaments are the 10 nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE: NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody can recognize both NEFH and NEFM.
Protocole
Product Specific Protocols | |
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WB protocol for NF-H/NF200 antibody 18934-1-AP | Download protocol |
IHC protocol for NF-H/NF200 antibody 18934-1-AP | Download protocol |
IF protocol for NF-H/NF200 antibody 18934-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Metab Gut microbiota modulate distal symmetric polyneuropathy in patients with diabetes | ||
Small Methods Polydopamine-Decorated PLCL Conduit to Induce Synergetic Effect of Electrical Stimulation and Topological Morphology for Peripheral Nerve Regeneration | ||
PLoS Genet Tracking the Fragile X Mental Retardation Protein in a Highly Ordered Neuronal RiboNucleoParticles Population: A Link between Stalled Polyribosomes and RNA Granules. | ||
Glia ST8SIA2 promotes oligodendrocyte differentiation and the integrity of myelin and axons. | ||
Int J Pharm Berberine-loaded MSC-derived sEVs encapsulated in injectable GelMA hydrogel for spinal cord injury repair |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH aidan (Verified Customer) (07-27-2024) | icc on retinal neurons (tried both mouse and human - human in pic). worked very well. would highly recommend
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FH Paula (Verified Customer) (04-17-2024) | Works well for immunocytochemistry. Cells after neurogenic differentiation - image attached.
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FH Azita (Verified Customer) (06-02-2021) | Western blotting analysis of NSC34 cells using NF-H Polyclonal Antibody showed strong bonds when the antibody was used at the dilution of 1:400.
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