Anticorps Polyclonal de lapin anti-MGP

• Phare
• Validé par KD/KO

MGP Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

35

Avis (1)

N° de cat : 10734-1-AP

Synonymes

GIG36, matrix Gla protein, MGLAP, MGP, NTI

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Galerie de données de validation

• 

  IHC staining of human colon cancer using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast hyperplasia using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10734-1-AP (MGP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10734-1-AP (MGP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10734-1-AP (MGP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10734-1-AP (MGP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 10734-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10734-1-AP (MGP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse kidney using 10734-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using MGP antibody (10734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse kidney using 10734-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using MGP antibody (10734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du sein humain, tissu de lymphome humain, tissu d'hyperplasie mammaire humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu rénal de souris,

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 23 publications below
• IHC: See 10 publications below
• IF: See 7 publications below

Informations sur le produit

10734-1-AP cible MGP dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MGP Protéine recombinante Ag1091
• Nom complet: matrix Gla protein
• Masse moléculaire calculée: 103 aa, 13 kDa
• Numéro d’acquisition GenBank: BC005272
• Symbole du gène: MGP
• Identification du gène (NCBI): 4256
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Matrix Gla protein (MGP), a vitamin K-dependent protein, is recognized as a calcification inhibitor in vascular tissue. Defects in MGP are the cause of Keutel syndrome (KS), which is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia.

Publications

Species	Application	Title
mouse	IHC	Circulation; Bone morphogenic protein antagonists are coexpressed with bone morphogenic protein 4 in endothelial cells exposed to unstable flow in vitro in mouse aortas and in human coronary arteries: role of bone morphogenic protein antagonists in inflammation and atherosclerosis.; Authors - Chang Kyunghwa K
mouse	WB	Nat Commun; Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease.; Authors - Mehdi Eshraghi
human	IHC	Arthritis Rheum; Juvenile dermatomyositis calcifications selectively displayed markers of bone formation.; Authors - Urganus Annette L AL
rat	WB	Aging (Albany NY); Intermedin1-53 attenuates aging-associated vascular calcification in rats by upregulating sirtuin 1.; Authors - Yao Chen
human	WB	FASEB J; 2,3,7,8-Tetrachlorodibenzo-p-dioxin promotes injury-induced vascular neointima formation in mice.; Authors - Shumin Guo
human	WB	Int J Obes (Lond); Using gene expression to predict the secretome of differentiating human preadipocytes.; Authors - Mutch D M DM

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kenzo (Verified Customer) (08-29-2023)

This antibody worked pretty well for IF on mouse liver frozen sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Liver

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