CHI3L1/YKL40 Polyclonal antibody

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CHI3L1/YKL40 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 12036-1-AP

Publications(45)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

CHI3L1, CGP 39, CGP-39, Chitinase 3 like protein 1, Chitinase-3-like protein 1

• 

  WB analysis of THP-1 using 12036-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 12036-1-AP (CHI3L1/YKL40 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of THP-1 using 12036-1-AP

  WB result of CHI3L1/YKL40 antibody (12036-1-AP; 1:10000; room temperature for 1.5 hours) with wild-type and CHI3L1/YKL40 knockout THP-1 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 12036-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12036-1-AP (CHI3L1/YKL40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 12036-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12036-1-AP (CHI3L1 Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 12036-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12036-1-AP (CHI3L1 Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 12036-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12036-1-AP (CHI3L1 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 12036-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12036-1-AP (CHI3L1 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse brain using 12036-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CHI3L1/YKL40 antibody (12036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse brain using 12036-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CHI3L1/YKL40 antibody (12036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of THP-1 using 12036-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CHI3L1/YKL40 antibody (12036-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: THP-1 cells, L02 cells
• Positive IHC detected in: human liver tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12036-1-AP targets CHI3L1/YKL40 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, rabbit, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2710

  Product name: Recombinant human CHI3L1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 34-383 aa of BC008568

  Sequence: YREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT

• Full Name: chitinase 3-like 1 (cartilage glycoprotein-39)
• Calculated Molecular Weight: 383 aa, 43 kDa
• Observed Molecular Weight: 40, 33 kDa
• GenBank Accession Number: BC008568
• Gene Symbol: CHI3L1
• Gene ID (NCBI): 1116
• RRID: AB_2877819
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P36222
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein. (PMID:21763261)

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Sci Bull (Beijing); Single-cell profile reveals the landscape of cardiac immunity and identifies a cardio-protective Ym-1hi neutrophil in myocardial ischemia-reperfusion injury; Authors - Yalan Dong
human	IF	Nat Commun; The immune landscape of human thymic epithelial tumors; Authors - Zhongwei Xin
	WB	J Nanobiotechnology; Secretome enriched with small extracellular vesicles derived from human gingiva-derived mesenchymal stem cells enhances rat tongue muscle regeneration; Authors - Qunzhou Zhang
human, mouse	WB	Adv Sci (Weinh); Nociceptive Nerve-Derived CGRP Exacerbates Uterine Fibrogenesis in Adenomyosis by Promoting CD140b(+) CD146(+) Fibroblast Differentiation.; Authors - Zi Ye
		Cell Rep Med; EGFR inhibition promotes functional engraftment and graft survival via donor-derived CHI3L1 in fetal RPE suspension transplantation.; Authors - Xiying Mao
mouse		Sci Adv; CHI3L1 signaling impairs hippocampal neurogenesis and cognitive function in autoimmune-mediated neuroinflammation; Authors - Wei Jiang