Osteocalcin Polyclonal antibody

Osteocalcin Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 20277-1-AP

Synonyms

Bglap, Bglap1, mOC A, OC, OG1, Osteocalcin

---

Validation Data Gallery

• 

  IHC staining of mouse skeletal muscle using 20277-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20277-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 20277-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20277-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 20277-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung using 20277-1-AP (Osteocalcin antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse lung using 20277-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung using 20277-1-AP (Osteocalcin antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive IHC detected in: mouse skeletal muscle tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

20277-1-AP targets Osteocalcin in WB, IHC, IF, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse, rat, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: bone gamma carboxyglutamate protein 1
• Calculated Molecular Weight: 11 kDa
• GenBank Accession Number: NM_007541
• Gene Symbol: Osteocalcin
• Gene ID (NCBI): 12096
• RRID: AB_11232596
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Osteocalcin (Bglap1), also named as Bglap2, Bglap, BGP, OC, OG1 and mOC-A, belongs to the osteocalcin/matrix Gla protein family. Bglap1 constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. The antibody is specific to mouse Bglap1.

Publications

Species	Application	Title
mouse	WB	Small; Honeycomb Bionic Graphene Oxide Quantum Dot/Layered Double Hydroxide Composite Nanocoating Promotes Osteoporotic Bone Regeneration via Activating Mitophagy; Authors - Dan Li
mouse,rabbit	IHC	Acta Biomater; A composite membrane with microtopographical morphology to regulate cellular behavior for improved tissue regeneration; Authors - Rui Zhang
mouse	IHC	Sci Rep; Experimental arthritis and Porphyromonas gingivalis administration synergistically decrease bone regeneration in femoral cortical defects.; Authors - Go Okumura
rat	IF	Microsyst Nanoeng; Enhancing metabolic activity and differentiation potential in adipose mesenchymal stem cells via high-resolution surface-acoustic-wave contactless patterning.; Authors - Karina Martinez Villegas
rabbit	IF	J Orthop Surg Res; Temporal transcriptome highlights the involvement of cytokine/JAK/STAT3 signaling pathway in the osteoinduction of BMSCs; Authors - Xiao Ru
mouse	WB	Eur J Med Res; CircZfp644-205 inhibits osteoblast differentiation and induces apoptosis of pre-osteoblasts via sponging miR-455-3p and promoting SMAD2 expression; Authors - Peng Zhang