Osteocalcin Polyclonal antibody

Osteocalcin Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20277-1-AP

Synonyms

Bglap, Bglap1, mOC A, OC, OG1, Osteocalcin



Tested Applications

Positive IHC detected inmouse skeletal muscle tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20277-1-AP targets Osteocalcin in WB, IHC, IF, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivitymouse, rat, rabbit
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name bone gamma carboxyglutamate protein 1
Calculated Molecular Weight 11 kDa
GenBank Accession NumberNM_007541
Gene Symbol Osteocalcin
Gene ID (NCBI) 12096
RRIDAB_11232596
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Osteocalcin (Bglap1), also named as Bglap2, Bglap, BGP, OC, OG1 and mOC-A, belongs to the osteocalcin/matrix Gla protein family. Bglap1 constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. The antibody is specific to mouse Bglap1.

Protocols

Product Specific Protocols
IHC protocol for Osteocalcin antibody 20277-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,rabbitIHC

Acta Biomater

A composite membrane with microtopographical morphology to regulate cellular behavior for improved tissue regeneration

Authors - Rui Zhang
mouseIHC

Sci Rep

Experimental arthritis and Porphyromonas gingivalis administration synergistically decrease bone regeneration in femoral cortical defects.

Authors - Go Okumura
ratIF

Microsyst Nanoeng

Enhancing metabolic activity and differentiation potential in adipose mesenchymal stem cells via high-resolution surface-acoustic-wave contactless patterning.

Authors - Karina Martinez Villegas
rabbitIF

J Orthop Surg Res

Temporal transcriptome highlights the involvement of cytokine/JAK/STAT3 signaling pathway in the osteoinduction of BMSCs

Authors - Xiao Ru
mouseWB

Eur J Med Res

CircZfp644-205 inhibits osteoblast differentiation and induces apoptosis of pre-osteoblasts via sponging miR-455-3p and promoting SMAD2 expression

Authors - Peng Zhang