Osteocalcin Polyclonal antibody
Osteocalcin Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 20277-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | mouse skeletal muscle tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
IHC | See 2 publications below |
IF | See 2 publications below |
Product Information
20277-1-AP targets Osteocalcin in WB, IHC, IF, ELISA applications and shows reactivity with mouse samples.
Tested Reactivity | mouse |
Cited Reactivity | mouse, rat, rabbit |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | bone gamma carboxyglutamate protein 1 |
Calculated Molecular Weight | 11 kDa |
GenBank Accession Number | NM_007541 |
Gene Symbol | Osteocalcin |
Gene ID (NCBI) | 12096 |
RRID | AB_11232596 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Osteocalcin (Bglap1), also named as Bglap2, Bglap, BGP, OC, OG1 and mOC-A, belongs to the osteocalcin/matrix Gla protein family. Bglap1 constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. The antibody is specific to mouse Bglap1.
Protocols
Product Specific Protocols | |
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IHC protocol for Osteocalcin antibody 20277-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Acta Biomater A composite membrane with microtopographical morphology to regulate cellular behavior for improved tissue regeneration | ||
Sci Rep Experimental arthritis and Porphyromonas gingivalis administration synergistically decrease bone regeneration in femoral cortical defects. | ||
Microsyst Nanoeng Enhancing metabolic activity and differentiation potential in adipose mesenchymal stem cells via high-resolution surface-acoustic-wave contactless patterning. | ||
J Orthop Surg Res Temporal transcriptome highlights the involvement of cytokine/JAK/STAT3 signaling pathway in the osteoinduction of BMSCs | ||
Eur J Med Res CircZfp644-205 inhibits osteoblast differentiation and induces apoptosis of pre-osteoblasts via sponging miR-455-3p and promoting SMAD2 expression |