Anticorps Polyclonal de lapin anti-MGEA5

• Phare
• Validé par KD/KO

MGEA5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA

Conjugaison

Non conjugué

Publications

71

Avis (1)

N° de cat : 14711-1-AP

Synonymes

OGA, Hexosaminidase C, HEXC, EC:3.2.1.169, Beta-N-acetylhexosaminidase

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Galerie de données de validation

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  WB analysis using 14711-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14711-1-AP (MGEA5 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14711-1-AP

  WB result of MGEA5 antibody (14711-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MGEA5 transfected HepG2 cells.

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  WB analysis using 14711-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14711-1-AP (MGEA5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 14711-1-AP

  IP result of anti-MGEA5 (IP:14711-1-AP, 4ug; Detection:14711-1-AP 1:2000) with HepG2 cells lysate 1880 ug.

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  IHC staining of human pancreas using 14711-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14711-1-AP (MGEA5 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 14711-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14711-1-AP (MGEA5 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human meningioma using 14711-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma slide using 14711-1-AP (MGEA5 Antibody) at dilution of 1:50.

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  IHC staining of human meningioma using 14711-1-AP

  Immunohistochemical analysis of paraffin-embedded human meningioma slide using 14711-1-AP (MGEA5 Antibody) at dilution of 1:50.

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  IHC staining of human pancreas using 14711-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 14711-1-AP (MGEA5 Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 14711-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MGEA5 antibody (14711-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 14711-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human MGEA5 (14711-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules SMMC-7721, cellules BxPC-3, cellules HepG2
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu pancréatique humain, tissu de méningiome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 59 publications below
• IHC: See 8 publications below
• IF: See 9 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

14711-1-AP cible MGEA5 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Drosophile, Humain, poisson-zèbre, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MGEA5 Protéine recombinante Ag6405
• Nom complet: meningioma expressed antigen 5 (hyaluronidase)
• Masse moléculaire calculée: 103 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC039583
• Symbole du gène: MGEA5
• Identification du gène (NCBI): 10724
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Human meningioma-expressed antigen 5 (MGEA5) has two putative domains including protein O-GlcNAcase domain and histone acetyltransferase domain, therefore it is often called bifunctional protein NCOAT. Three isoforms of MEGE5 are produced by alternative splicing. MGEA5 was found to be regulated to reduce the state of glycosylation of transcitpional activators while increasing the acetylation of histones to allow for the concerted activation of eukaryotic gene transcription, for instance, acetylation of Lys8 of histone H4 and Lys 14 of histone H3 are resulted from acetyltransferase activity. In addition, single nucleotide polymorphism in MGE5A is associated with type 2 diabetes in Mexican Americans. Two bands at 130kDa and 75 kDa could be detected using the present rabbit polyclonal antibody 14711-1-AP, which is consistent with results in a related reference (PubMed:11341771).

Publications

Species	Application	Title
human	WB	Nat Cell Biol; O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins; Authors - Yulin Chen
human	WB	J Clin Invest; Cancer-associated fibroblast-secreted glucosamine alters the androgen biosynthesis program in prostate cancer via HSD3B1 upregulation; Authors - Di Cui
human	IHC	Biomaterials; PKM2 allosteric converter: A self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy; Authors - Lu Wang
rat	WB	Redox Biol; Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation.; Authors - Wei Ou
mouse	WB	Proc Natl Acad Sci U S A; Polycomb repressive complex 2 is necessary for the normal site-specific O-GlcNAc distribution in mouse embryonic stem cells.; Authors - Myers Samuel A SA
Drosophila	WB	Proc Natl Acad Sci U S A; O-GlcNAc reports ambient temperature and confers heat resistance on ectotherm development.; Authors - Pablo T Radermacher

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wei (Verified Customer) (02-28-2019)

Always two bands together

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12

MGEA5 Antibody Western Blot, validation (1:1000 dilution) in C2C12 (Cat no:14711-1-AP)

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